We narrowed to 9,611 results for: bli

1. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

   Plasmid

   #183606

   Purpose

   Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Tags

   HA, Human PDGFRB transmembrane domain, Mouse IgGK…

   Expression

   Mammalian

   Promoter

   Mouse Pgk1

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28sumo-MDA5-Full-length

   Plasmid

   #210826

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

   Insert

   MDA5 (IFIH1) Full Length Protein (IFIH1 Human)
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-HA-mRFP-pcDNA3.1-

   Plasmid

   #52510

   Purpose

   expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   mRFP
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   engineered variant of tetrameric red fluorescent …

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETNKI-strepII-3C-LIC-Amp

   Plasmid

   #108707

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-FHC-POLQ-DM

   Plasmid

   #64880

   Purpose

   lentiviral expression of human POLQ double mutant (in POL and HDL domains)

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   K121M and D2330A,Y2331A

   Promoter

   EF1alpha

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASp YFP Ras85D CA

   Plasmid

   #52247

   Purpose

   Drosophila expression of YFP-Ras85D constitutively active mutant

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q61L; CA mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pMXs-hFOXH1

   Plasmid

   #59183

   Purpose

   Forced expression of human FOXH1

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

   Insert

   FOXH1 (FOXH1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. SNX29-PX (269-391)

   Plasmid

   #119108

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX29-PX (269-391)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_HcRed

   Plasmid

   #25892

   Purpose

   Gateway entry vector containing HcRed.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   hcRed
   

   Use

   Gateway entry vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid

    #218778

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(3)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p11d-tRPA-32Ala9

    Plasmid

    #102616

    Purpose

    Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).

    Depositor

    Marc Wold

    Inserts

    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    

    Expression

    Bacterial

    Mutation

    with an alanine substituted phosphorylation domain

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFE-Selo-FI-prk

    Plasmid

    #162698

    Purpose

    Expresses S. elongatus Form IB rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    S. elongatus Form IB rubisco and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid

    #122964

    Purpose

    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Insert

    human Chibby1 (CBY1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Promoter

    FoxJ1 and human FoxJ1

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET14b-dabAB2

    Plasmid

    #133013

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUb-tdTomato-LP-Neo-LP

    Plasmid

    #231509

    Purpose

    Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system

    Depositor

    Yeonchul Hong

    Insert

    tdTomato fluorescent protein
    

    Use

    Unspecified; Acanthamoeba castellanii neff strain

    Promoter

    Acanthamoeba polyubiquitin

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAPDH-Cre-HYG

    Plasmid

    #231510

    Purpose

    Expresses Cre recombinase in Acanthamoeba

    Depositor

    Yeonchul Hong

    Insert

    Cre recombinase
    

    Use

    Unspecified; Acanthamoeba castellanii neff strain

    Tags

    EGFP and SV40 NLS

    Promoter

    Acanthamoeba GAPDH

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQTEV-CDO1

    Plasmid

    #31292

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    cysteine dioxygenase, type I (CDO1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-Lck-FRB-mTurquoise2

    Plasmid

    #176130

    Purpose

    Plasma membrane localized FRB, rapamycin heterodimerization

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    FRB
    

    Use

    Lentiviral

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFE-Rrub-FII-prk

    Plasmid

    #162695

    Purpose

    Expresses R. rubrum Form II rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Inserts

    Rubrum Form II rubisco
    S. elongatus PRK
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter and under same promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid

    #53225

    Purpose

    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

    Depositor

    Jesse Rinehart

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Phosphoprotein synthesis

    Tags

    HIS and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli, both codons for Ser21…

    Promoter

    pLtetO

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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