We narrowed to 4,243 results for: Pet2

1. pET28a-MBP-(CBX1)3-eReader

   Plasmid

   #135090

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered x3
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-His6-Ubc9-C138A

   Plasmid

   #133911

   Purpose

   Bacterial expression of His6-Ubc9-C138A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 138 to Alanine

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-TAF4(827-909)

   Plasmid

   #105620

   Purpose

   bacterially express TAF4 HFD

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   TAF4-HFD(827-909) (Taf4 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Pet22b-mCherry-TEAD4-IDR

   Plasmid

   #166442

   Purpose

   Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mCherry, TEAD4-IDR (TEAD4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28A-p53 (1-73) (P12A P13A)

   Plasmid

   #62066

   Purpose

   Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains TAD residues 1-73; P12A, P13A

   Promoter

   T7

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET21a-mAIM2-deltaPYD-GST

   Plasmid

   #51534

   Purpose

   Express GST-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Use

   Tags

   GST and T7

   Expression

   Bacterial

   Mutation

   Promoter

   lac operator

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET22b-ecDHFR-N23PP/G51PEKN

   Plasmid

   #118583

   Purpose

   Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, G51 mutated to P51, a Pro res…

   Promoter

   T7

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET27b-ecDHFR-N23PP/S148A

   Plasmid

   #110829

   Purpose

   Bacterial expression of E.coli DHFR N23PP/S148A mutant

   Depositor

   Stephen Benkovic

   Article

   Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

   Insert

   ecDHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23; a proline residue inserted be…

   Promoter

   T7

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-Hisx6-Sirt6-S56A

   Plasmid

   #20278

   Depositor

   Leonard Guarente

   Article

   Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

   Insert

   Sirt6 (Sirt6 Mouse)
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Serine 56 to Alanine

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pET28-Nsp14-TEV-Strep

    Plasmid

    #154953

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pET22-Strep-TEV-Nsp10

    Plasmid

    #154958

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Strep-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pET21a-hAIM2-PYD-GST

    Plasmid

    #51531

    Purpose

    Express GST-tagged PYD of hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    GST and T7

    Expression

    Bacterial

    Mutation

    deletion of amino acids 102-343

    Promoter

    lac operator

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28b-NmCas9-Flag H588A

    Plasmid

    #109032

    Purpose

    H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET22b-EC12-129/138

    Plasmid

    #47926

    Purpose

    Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    Mutations C9A, K129C, D138C

    Promoter

    T7

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28 His-SUMO-CHMP2B

    Plasmid

    #232018

    Purpose

    Bacterial expression of His and SUMO tagged CHMP2B

    Depositor

    Franz-Ulrich Hartl

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Tags

    6x His, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-His6-UROD-N367A

    Plasmid

    #236740

    Purpose

    Protein overexpression of human uroporphyrinogen decarboxylase N367A

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    Uroporphyrinogen decarboxylase human N367A (UROD Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N367A

    Promoter

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-His6-GFP-UROD

    Plasmid

    #236741

    Purpose

    Protein overexpression of eGFP-human uroporphyrinogen decarboxylase

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    eGFP-Uroporphyrinogen decarboxylase human (UROD Synthetic, Human)
    

    Use

    Tags

    His6 and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a asyn(Δ103-130)

    Plasmid

    #242235

    Purpose

    Expresses alpha synuclein (missing exon 5, residues 103-130) in bacteria

    Depositor

    Jennifer Lee

    Article

    SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

    Insert

    asyn(Δ103-130)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    deletion of residues 103-130

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a asyn(66-140) E83A

    Plasmid

    #240583

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83A

    Promoter

    T7

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a asyn(66-140) K80A

    Plasmid

    #240584

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K80A

    Promoter

    T7

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21a asyn(66-140) K96A

    Plasmid

    #240585

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K96A

    Promoter

    T7

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21a asyn(66-140) K97A

    Plasmid

    #240586

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K97A

    Promoter

    T7

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a asyn(66-140) K102A

    Plasmid

    #240588

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K102A

    Promoter

    T7

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21a asyn(66-140) D98A

    Plasmid

    #240587

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, D98A

    Promoter

    T7

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET23a-His-TEV-SpyCatcher3

    Plasmid

    #235149

    Purpose

    Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET29_anti-SARS-CoV-2-NM1226

    Plasmid

    #209422

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1226
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pET29_anti-SARS-CoV-2-NM1230

    Plasmid

    #209423

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1230
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pET29_anti-MERS-Nanobody-VHH-1

    Plasmid

    #206890

    Purpose

    Express anti-MERS-Nanobody-VHH-1 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pET29_anti-MERS-Nanobody-VHH-55

    Plasmid

    #209417

    Purpose

    Express anti-MERS-Nanobody-VHH-55 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-55
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pET29_anti-MERS-Nanobody-VHH-83

    Plasmid

    #209418

    Purpose

    express anti-MERS-Nanobody-VHH-83 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-83
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pET29_anti-MERS-Nanobody-VHH-84

    Plasmid

    #209419

    Purpose

    Express anti-MERS-Nanobody-VHH-84 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-83
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pET29_anti-SARS-CoV-2-Nanobody-C5

    Plasmid

    #209420

    Purpose

    Express anti-SARS-CoV-2-Nanobody C5 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-C5
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pET29_anti-SARS-CoV-2-Nanobody-F2

    Plasmid

    #209421

    Purpose

    Express anti-SARS-CoV-2-Nanobody F2 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-F2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pET28b-SUMO-FLAG-TeKaiC

    Plasmid

    #229222

    Purpose

    For expression of T. elongatus KaiC protein

    Depositor

    Andy LiWang

    Insert

    kaiC (kaiC T. elongatus)
    

    Use

    Tags

    SUMO-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28b-SUMO-SeKaiB-FLAG

    Plasmid

    #229219

    Purpose

    For expression of S. elongatus KaiB protein

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB S. elongatus)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-hfYFP-TEV-Bcx

    Plasmid

    #191195

    Purpose

    Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Bcx fusion
    

    Use

    Tags

    6xHis and Bacillus circulans xylanase

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-hfYFP-TEV-mScarlet-i

    Plasmid

    #191196

    Purpose

    Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & mScarlet-I fusion
    

    Use

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-hfYFP-TEV-SAV

    Plasmid

    #191197

    Purpose

    Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-LSSmGFP-TEV-mScarlet-i

    Plasmid

    #191199

    Purpose

    Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & mScarlet-I fusion
    

    Use

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-LSSmGFP-TEV-SAV

    Plasmid

    #191200

    Purpose

    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge