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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,550 results for: 187

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Showing: 801 - 820 of 4550 results

  2. pKP2187

    Plasmid
    #167640
    Purpose
    Express FepA V15C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA V15C, I14P
    Expression
    Bacterial
    Mutation
    fepA with V15C, I14P mutations (Mutations are I36…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167640-plasmid-map-sequence-id-339573

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  9. Epac-S-H187

    Plasmid
    #170348
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170348-plasmid-map-sequence-id-336137

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  12. pCDZ2FE_Rev3_1873-2025_4PA

    Plasmid
    #105673
    Purpose
    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Rev3L (REV3L Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P1880A, P1885A, P1996A, P2001A
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105673-plasmid-map-sequence-id-203204

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  14. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  15. pGBW-m4046187

    Plasmid
    #145403
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 leader protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 leader protein (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145403-plasmid-map-sequence-id-298845

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  19. pCDZ2FE_Rev3_1873-2025

    Plasmid
    #105672
    Purpose
    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein
    Depositor
    Thanos Halazonetis
    Insert
    Rev3L (REV3L Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105672-plasmid-map-sequence-id-203200

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  20. pGBW-m4134187

    Plasmid
    #152565
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 helicase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152565-plasmid-map-sequence-id-295577

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Showing: 801 - 820 of 4550 results