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Plasmid#105193PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-CCL21-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN428 (FuGW-G5p-mKate2-1Pe )
Plasmid#105184PurposeModule 3 - GAL4 synthetic promoter (with five binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)DepositorInsertG5p-mKate2-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLN434 (FuGW-G14p-mKate2-1Pe )
Plasmid#105188PurposeModule 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)DepositorInsertG14p-mKate2-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z5-Nluc
Plasmid#174487PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ5-Nluc
UseTags6x Histidine tag (N terminal on insert)ExpressionBacterialMutationPromoterT7lac promoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin5
Plasmid#41916DepositorInsertCUL5 (CUL5 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-RNF2_+5GtoT
Plasmid#135957PurposeS. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editingDepositorInsertRNF2_5GtoT pegRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationWTPromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES_EGFP_GA_KCNE1
Plasmid#173160PurposeFor mammalian expression of KCNE1 and creating variantsDepositorInsertKCNE1 (KCNE1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pYES260-sc.eEF2
Plasmid#85119Purposeyeast expression of EEF2DepositorUseTags6xHisExpressionYeastMutationPromoterGAL1Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/Venus-mATG5
Plasmid#58739PurposeExpresses Venus-mATG5 in mammalian cellsDepositorInsertautophagy related genes 5 (Atg5 Mouse)
UseRetroviralTagsVenusExpressionMutationPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/SECFP-mATG5
Plasmid#58744PurposeExpresses /SECFP-ATG5 in mammalian cellsDepositorInsertautophagy related genes 5 (Atg5 Mouse)
UseRetroviralTagsSECFPExpressionMutationPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-EGFP-HRasC20
Plasmid#187252PurposeLenticiral expression of EGFP-HRasC20DepositorInsertHRasC20 (HRAS Human)
UseLentiviralTagsEGFPExpressionMutationPromoter5' LTRAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-loxP-canisNrg1
Plasmid#173849PurposeEncoding dog Nrg1 before Cre-mediated recombination.DepositorInsertA cDNA of canis Nrg1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEgf
Plasmid#173700PurposeA knock-out vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-neo-dTgfa
Plasmid#173842PurposeA knockout vector for the dog Tgfa.DepositorInsertA gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-puro-dEgfr
Plasmid#173844PurposeA knockout vector for the dog Egfr.DepositorInsertA gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisEgf
Plasmid#173845PurposeEncoding dog Egf.DepositorInsertA cDNA of canis EGF.
UseTagsExpressionMammalianMutationR1127Q- please see dep. commentsPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only