We narrowed to 13,452 results for: Mpl;

1. pPpT4- TEF2prom-TIR1-FLAG

   Plasmid

   #189726

   Purpose

   Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   TEF2

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. L1_NOP1:mS-N7-Ck2

   Plasmid

   #136126

   Purpose

   L1 in position 2, example of weak promoter

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpNOP1:mScarletI-N7_3tNos-35S
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pUC19 gene 60 thru gap + Cys

   Plasmid

   #49161

   Purpose

   Template for generating gene 60 mRNA truncated two codons after coding gap + Cys

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Mutation

   gene 60 mRNA truncated two codons after coding ga…

   Promoter

   T7

   Available Since

   Feb. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVM_02

   Plasmid

   #204500

   Purpose

   Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   amino acids 54-213 VHL (VHL Human)
   

   Tags

   6xHis, TEV, and Thrombin

   Expression

   Bacterial

   Mutation

   0

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-TtCas6-4xcrRNA4.5

   Plasmid

   #127764

   Purpose

   For expression of T. thermophilus Csm complex in E. coli (crRNA)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Synthetic CRISPR array containing four identical T. thermophilus spacers
   Cas6A
   

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-AKAR4-NLS

   Plasmid

   #138217

   Purpose

   Nuclear-targeted FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   AKAR4-NLS
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. STAGR_gRNAScaffold_mU6

   Plasmid

   #102844

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert gRNAScaffold_mU6
   

   Use

   Pcr template for stagr inserts

   Promoter

   mU6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bmi1-overexpression

   Plasmid

   #21577

   Depositor

   Sally Temple

   Article

   Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

   Insert

   Bmi-1 (Bmi1 Mouse)
   

   Use

   Lentiviral

   Tags

   ires-eGFP

   Expression

   Mammalian

   Available Since

   June 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. STAGR_gRNAScaffold_hH1

   Plasmid

   #102841

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert gRNAScaffold_hH1
   

   Use

   Pcr template for stagr inserts

   Promoter

   hH1

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bmi1 shRNA1

    Plasmid

    #21576

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Insert

    Bmi-1 (Bmi1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-OSF-VPS15

    Plasmid

    #99326

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    VPS15 (PIK3R4 Human)
    

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-ABKAR

    Plasmid

    #140051

    Purpose

    Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. STAGR_SAMScaffold_hU6

    Plasmid

    #102843

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_hU6promoter
    

    Use

    As pcr template

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAdSh.U6.gRNAGFP

    Plasmid

    #58255

    Purpose

    U6 promoter-driven single guide RNA complementary to eGFP

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAGFP cassette
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXWJ109AsAmp30E

    Plasmid

    #123144

    Purpose

    Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, ParsR, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2SCb

    Plasmid

    #59385

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat2
    

    Use

    Template

    Mutation

    The 3’-end of the chloramphenicol resistance gene…

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAdSh.U6.gRNAS1

    Plasmid

    #58252

    Purpose

    U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAS1 cassette
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXWJ109HgAmp30E

    Plasmid

    #123149

    Purpose

    Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, PmerT, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. STAGR_SAMScaffold_mU6

    Plasmid

    #102845

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_mU6
    

    Use

    Pcr template for stagr inserts

    Promoter

    mU6

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tol2pA2-drl:APEX2-mCherry

    Plasmid

    #188945

    Purpose

    Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

    Depositor

    Owen Tamplin

    Article

    Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

    Insert

    drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
    

    Use

    Tol2 destination vector (attr4-r3)

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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