We narrowed to 2,170 results for: Pam

1. SK-YFP-TD-B

   Plasmid

   #48663

   Purpose

   Bacterial TD repression YFP reporter: protospacer B

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   TD prototspacer B/PAM with reporter EYFP
   

   Use

   CRISPR

   Promoter

   lambda pR promoter

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. SK-YFP-ST1-A

   Plasmid

   #48665

   Purpose

   Bacterial ST1 repression YFP reporter: protospacer A

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   ST1 prototspacer A/PAM with reporter EYFP
   

   Use

   CRISPR

   Promoter

   lambda pR promoter

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. SK-YFP-ST1-B

   Plasmid

   #48662

   Purpose

   Bacterial ST1 repression YFP reporter: protospacer B

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   ST1 prototspacer B/PAM with reporter EYFP
   

   Use

   CRISPR

   Promoter

   lambda pR promoter

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKO023

   Plasmid

   #242923

   Purpose

   Reporter J1-J3(122)-BBa_J23117 with Sth1 PAM

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

   Insert

   J1-J3(122)
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   Bba_J23117

   Available Since

   Sept. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CRISPR-Puro-sgRPL3-62

   Plasmid

   #208647

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CRISPR-Puro-sgRPL3-56

   Plasmid

   #208979

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL1_3

   Plasmid

   #199035

   Purpose

   Level 1 plasmid, barcoded ORI

   Depositor

   Cultivarium Tools, Charlie Gilbert, Nili Ostrov

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAM?1 ORI plus NGS barcode 3
   

   Use

   Synthetic Biology

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPR-psbA2 point mutation

   Plasmid

   #182929

   Purpose

   point mutation on psbA2 by CRISPR in Synechocystis 6803

   Depositor

   Himadri Pakrasi

   Article

   Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

   Inserts

   ddcpf1
   gRNA targeting psbA2 in Synechocystis 6803: gatcttcggtcgcttgatctttc
   psbA
   

   Use

   CRISPR

   Mutation

   S264A, and silent mutation to remove PAM site

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SK-YFP-NM-A

   Plasmid

   #48664

   Purpose

   Bacterial NM repression YFP reporter: protospacer A

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   NM prototspacer A/PAM with reporter EYFP
   

   Use

   CRISPR

   Promoter

   lambda pR promoter

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SK-YFP-TD-A

    Plasmid

    #48666

    Purpose

    Bacterial TD repression YFP reporter: protospacer A

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TD prototspacer A/PAM with reporter EYFP
    

    Use

    CRISPR

    Promoter

    lambda pR promoter

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-DTR-GFP

    Plasmid

    #124364

    Purpose

    conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

    Depositor

    Thomas Jessell, Eiman Azim

    Article

    Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

    Has Service

    AAV2

    Insert

    DTR-GFP
    

    Use

    AAV

    Tags

    GFP

    Promoter

    Chicken B-actin

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9/VRQR-sgRNA (BbsI)

    Plasmid

    #129725

    Purpose

    Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS13: pTarget(AvCAST)

    Plasmid

    #168146

    Purpose

    Target plasmid containing AvPSP1 and attachement site for AvCAST.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PAM for AvCAST + AvPSP1
    glmS
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. px458 EQR

    Plasmid

    #101731

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. M-tdTom-SP

    Plasmid

    #48677

    Purpose

    Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9
    

    Use

    CRISPR

    Promoter

    Sp6

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

18. px330 VRER

    Plasmid

    #101729

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv2 sgSp1

    Plasmid

    #174907

    Purpose

    Second generation sgRNA against Sp1

    Depositor

    Jane Clifford

    Article

    Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

    Insert

    Specificity Protein 1 (SP1 Human)
    

    Expression

    Mammalian

    Mutation

    Missense mutation to mutate PAM sequence

    Promoter

    EFS-NS

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge