Addgene

We narrowed to 1,639 results for: Tyr

Showing: 801 - 820 of 1639 results

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pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

Plasmid

#201548

Purpose

Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks C-terminal residues 364-409

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G)

Plasmid

#180029

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)

Use

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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Dr-FGFR1

Plasmid

#184005

Purpose

Dr-FGFR expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

Insert

Dr-FGFR1 (FGFR1 Human)

Use

Tags

Deinococcus radiodurans bacterial phytochrome

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLL5.0 CortW22A mCherry

Plasmid

#187268

Purpose

Lenticiral expression of mCherry-Cortactin W22A

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

W22A

Promoter

5' LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

Y531F

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_withR

Plasmid

#139128

Purpose

express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

add arginines

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_nocharge_wR

Plasmid

#139119

Purpose

express hnRNPA2 LC with no charged residues with R added back

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

remove all K, most D

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) R104A

Plasmid

#125826

Purpose

Expresses GST-RPN13 (19-132) R104A in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to alanine

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) R104D

Plasmid

#125827

Purpose

Expresses GST-RPN13 (19-132) R104D in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to aspartate

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) R104E

Plasmid

#125828

Purpose

Expresses GST-RPN13 (19-132) R104E in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to glutamate

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 Y158F

Plasmid

#123624

Purpose

Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Tyr158Phe

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-hAXL

Plasmid

#124797

Purpose

Tet-inducible AXL expression in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A_deltaSIV

Plasmid

#145771

Purpose

expresses human Nav1.5 lacking the SIV motif in mammalian cells

Depositor

Hugues Abriel

Article

Jespersen et al Biochem Biophys Res Commun. 2006 Oct 6;3

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

none

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRYc-ABE8e-EGFP

Plasmid

#208340

Purpose

Expresses SpRYc-ABE8e in mammalian cells

Depositor

Pranam Chatterjee

Article

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

ecTadA(8e)-nSpRYc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CMV

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-gateway

Plasmid

#51070

Purpose

gateway adapted mammalian expression vector

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His beta4

Plasmid

#16039

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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