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Plasmid#214983PurposeCytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).DepositorInsertAPEX2 (APX2 Glycine max (soybean))
UseLentiviralTags3xFLAGExpressionMutationPromoterUBCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKB231
Plasmid#170018PurposeEncodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Cecropin P1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
EphA1-RTK-Cry2olig
Plasmid#176601PurposeEphrinA1 RTK fusion to Cry2olig - RTK domain onlyDepositorInsertEphA1rtk-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV2cap
Plasmid#198014PurposeFor high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV2 cap
UseAAVTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matMYC
Plasmid#115248PurposeFor mammalian expression of the human NODAL open reading frame with an internal MYC tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
stblHIF: pLenti-PGK-stblHIF-PGK-Puro
Plasmid#177202Purposeencoding stblHIF under PGK promoterDepositorArticleInsertstblHIF
UseLentiviralTagsExpressionMammalianMutationP402A/P577G/N813APromoterPGKAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iPASnFR-L
Plasmid#225236PurposeAAV expression of iPASnFR-L from hSyn promoterDepositorInsertiPASnFR-L
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::mCherry-H11
Plasmid#211940Purposedual-enSERT-1 mCherry vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHSP68Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH-roGFP2-synORP1
Plasmid#191680PurposeTi plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.DepositorInsertrredox(roGFP2-synORP1
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLDV_41 [11o1G]
Plasmid#199070PurposeOdd assembly vector with sfGFP dropout for Odd level assembliesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p007ampGFP
Plasmid#58535PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-RFX5
Plasmid#222545PurposePiggyBac transposon plasmid for doxycycline inducible expression of RFX5DepositorInsertRFX5 (RFX5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-SYMPK
Plasmid#222556PurposePiggyBac transposon plasmid for doxycycline inducible expression of SYMPKDepositorInsertSYMPK (SYMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT21
Plasmid#204309PurposeFor generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthpartsDepositorInsertie1-GFPn
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT25
Plasmid#204310PurposeFor generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.DepositorInsertie1-GFPc
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJYDC3
Plasmid#162460PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA designed ALFA nanobody, HA tag, HDEL sequ…ExpressionMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-ADRA2A
Plasmid#190753PurposeExpression of ADRA2A with mEGFP tag on N-terminus.DepositorInsertADRA2A (ADRA2A Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB222
Plasmid#170012PurposeEncodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
LL-37
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-ZCWPW1
Plasmid#222543PurposePiggyBac transposon plasmid for doxycycline inducible expression of ZCWPW1DepositorInsertZCWPW1 (ZCWPW1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mScarlet HDR template
Plasmid#214998PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only