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Plasmid#139502PurposeExpresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-mScarlet
Plasmid#139503PurposeExpresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsmScarlet following Gly-Gly-Ser linkerExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLCKO2::TKOv3
Pooled Library#125517PurposeHuman CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backboneDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
EcMscS
Plasmid#78555PurposeE. coli Mechanosensitive Channel of Small ConductanceDepositorInsertMechanosensitive Channel of Small Conductance
UseTags6x Histidine TagExpressionMutationPromoterT7 PromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus
Plasmid#139505PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV8 and AAV9Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-PPO-Venus
Plasmid#139504PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV5Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 hPHGDH
Plasmid#154916PurposeDoxycyclin inducible expression of human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST PHGDH
Plasmid#154917PurposeExpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiP13584 - pAAV-AiE0475m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3584)
Plasmid#224031PurposeAiE0475m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Chandelier cellsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP12782 - pAAV-AiE0476m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2782)
Plasmid#224005PurposeAiE0476m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Chandelier cells (note HPF ChC cells)InsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Toronto KnockOut version 3 library (TKOv3)
Pooled Library#90294PurposeOptimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1DepositorAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST co hPHGDH
Plasmid#154915PurposeExpress codon optimized human PHGDHDepositorInsertPHGDH, codon optimized (PHGDH Human, Synthetic)
UseRetroviralTagsExpressionMammalianMutationCodon optimized for expression in human cellsPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNC1124
Plasmid#41560DepositorInsertUra3-GAL1-UBIYdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNC1125
Plasmid#41561DepositorInsertUra3-GAL1-UBIMdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNC1136
Plasmid#41562DepositorInsertUra3-FUS1-UBIYdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNC1137
Plasmid#41563DepositorInsertUra3-FUS1-UBIMdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-C. thermophilum LIC
Plasmid#74780Purposeencodes GST-tagged dynein light intermediate chain from Chaetomium thermophilumDepositorInsertdynein light intermediate chain (Chaetomium thermophilum)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACAGW-ChR2-Venus-AAV (AAV1)
Viral Prep#20071-AAV1PurposeReady-to-use AAV1 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGGTagsVenusAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACAGW-ChR2-Venus-AAV (AAV9)
Viral Prep#20071-AAV9PurposeReady-to-use AAV9 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGGTagsVenusAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psCDK5
Plasmid#89365PurposeA lenti-viral vector expressing photoswitchable CDK5 in mammalian cellsDepositorInsertpsCDK5 (CDK5 Human, Synthetic)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only