We narrowed to 39,244 results for: ELL

Showing: 8181 - 8200 of 39244 results

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PL-5LTR-GW-A

Plasmid

#138480

Purpose

For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-AmCyan

Plasmid

#138481

Purpose

For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

AmCyan

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDM_T7_HisLacZomega

Plasmid

#118815

Purpose

T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

Depositor

Alexander Green

Publication

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

lacZ_

Tags

histag

Expression

Bacterial

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-6xHis-(pos15)GFP

Plasmid

#89248

Purpose

[Edit] Expresses GFP with +15 theoretical charge in E.Coli

Depositor

David Liu

Publication

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos15)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Tags

none

Expression

Mammalian

Promoter

CMV

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 merlin 308-595

Plasmid

#11628

Depositor

Vijaya Ramesh

Publication

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Carboxy-terminal half of merlin isoform 1.

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
wtBxb1_IVT

Plasmid

#222340

Purpose

Plasmid for wild type Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized wtBxb1

Use

Template for in vitro transcription

Promoter

T7 (inactivated)

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDD425

Plasmid

#202078

Purpose

Frame +0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Publication

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +0 sgRNA

Use

CRISPR

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDD427

Plasmid

#202080

Purpose

Frame +2/-1 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Publication

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA

Use

CRISPR

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSL3713 (pTnsC_Pse)

Plasmid

#200900

Purpose

Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

Depositor

Samuel H. Sternberg

Publication

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseTnsC

Tags

3xNLS

Expression

Mammalian

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-FLEX-SomaGCaMP7

Plasmid

#182942

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ GCamp6-3NLS

Plasmid

#165401

Purpose

Expresses a nuclear localized genetically encoded calcium reporter.

Depositor

Philipp Niethammer

Publication

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

GCamp6

Expression

Mammalian

Promoter

SP6

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_C_AOX

Plasmid

#111661

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AOX

Expression

Mammalian

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Construct 9 - U6p_30nt_stgRNA

Plasmid

#81248

Purpose

Expresses 30nt stgRNA

Depositor

Timothy Lu

Publication

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) TFRC

Plasmid

#74709

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

Depositor

Eugene Yeo

Publication

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only
pCAG tubulin GFP

Plasmid

#66105

Purpose

expression of chicken tubulin GFP

Depositor

Felicitas Proels, Martin Scaal

Publication

et al Development. 2015 Feb 15;142(4):665-71.

Insert

tubulin

Use

Chicken expression

Tags

EGFP

Promoter

CAG

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

Plasmid

#62956

Purpose

Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Publication

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-mLmo2

Plasmid

#64893

Purpose

Lentiviral expression of Flag-tagged murine transcription factor Lmo2

Depositor

Georges Lacaud

Publication

Batta et al Cell Rep. 2014 Dec 11;9(5):1871-84. doi:

Insert

Lmo2 (Lmo2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RIP7-RLuc-YFP-pBS

Plasmid

#72482

Purpose

Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals

Depositor

Vincent Poitout

Publication

Ghislain et al Endocrinology. 2015 Dec 15:en20151550.

Inserts

rat ins2 gene promoter (Ins2 Rat)
luciferase
eYFP

Use

Luciferase

Tags

eYFP and renilla luciferase

Expression

Mammalian

Mutation

eYFP varient

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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-53 bp human IFN-gamma luc

Plasmid

#17597

Depositor

Howard Young

Publication

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-53 to +70 of promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only