We narrowed to 22,846 results for: Tes

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PLK1-cjun-FRET-PACT biosensor

Plasmid

#106907

Purpose

centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)

Tags

PACT domain

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pIVT/Smarca4

Plasmid

#122146

Purpose

For making Smarca4 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarca4 (Smarca4 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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Donor-PIGA

Plasmid

#153530

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 1,991-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-ndufs2 guide

Plasmid

#107601

Purpose

An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

ndufs2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS2B

Plasmid

#120819

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS2B ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CHICA (Nigg HS342)

Plasmid

#41153

Depositor

Erich Nigg

Article

Santamaria et al Curr Biol. 2008 May 20;18(10):723-9.

Insert

CHICA (FAM83D Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

V11L

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDUAL_tacI

Plasmid

#155170

Purpose

BaSP and CuCBP co-expression: tacI promoter controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMALB

Plasmid

#161784

Purpose

Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and hPGK

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GRK2 S670D

Plasmid

#45756

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 S670D (GRK2 Bovine)

Expression

Mammalian

Mutation

S670D

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pENTR223-BZLF2

Plasmid

#195784

Purpose

Gateway Entry Clone of Epstein-Barr virus BZLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BZLF2 (BZLF2 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL

Plasmid

#161783

Purpose

Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and GFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and hPGK

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-EVE-MM

Plasmid

#22994

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A, S463E, S465E

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-Akt(K179M)

Plasmid

#39535

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(K179M) (Akt1 Mouse)

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

K179M

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-KG-CAND1

Plasmid

#19949

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pMSCVpuro BRIT1

Plasmid

#16205

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

BRIT1 (MCPH1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
p8103 LentiCRISPR v2 sgNT-2

Plasmid

#163313

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

sgNT-2

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRGECO2

Plasmid

#125244

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Expression

Mammalian

Mutation

W44Y, D432A

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNALP

Plasmid

#195817

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNALP

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNALP (EBNA-LP Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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