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PX458_KDM6A_1

Plasmid

#86307

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-OCT4

Plasmid

#69537

Purpose

Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

Depositor

Timo Otonkoski

Publication

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5 concatenated gRNA transcriptional cassettes targeting OCT4

Use

CRISPR

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD

Plasmid

#235574

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

Depositor

Jamie Hackett

Publication

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSUP-(2xCouRS-tRNAopt)

Plasmid

#197930

Purpose

Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

Depositor

Kenneth Johnson

Publication

Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

Inserts

CouRS
tRNA opt

Expression

Bacterial

Promoter

glnS' and lambda(ts) and proK

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-MjDim1

Plasmid

#189936

Purpose

Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

Depositor

Chuan He

Publication

Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

Insert

rsmA (MJ_RS00455 Methanocaldococcus jannaschii)

Tags

6x His tag, FLAG tag, SUMO tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TDG004_pLenti_CMVtight_Kir2.1_CFP

Plasmid

#178820

Purpose

tet-on expression of an inward-rectifier potassium channel Kir2.1 carrying an CFP tag

Depositor

Adam Cohen

Publication

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

Kir2.1_CFP (Kcnj2 Mouse)

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Trim46

Plasmid

#176402

Purpose

TRIM46 tagged with GFP

Depositor

Casper Hoogenraad, Thomas Schwarz

Publication

van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

trim46 (Trim46 Mouse)

Tags

GFP

Expression

Mammalian

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET24_NusA-Tus

Plasmid

#165959

Purpose

Expression of soluble E. coli Tus protein

Depositor

Keith Pardee

Publication

Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

Insert

Tus

Tags

His-NusA-Tev

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A hu_ncOGT WT

Plasmid

#154281

Purpose

Entry vector encoding non-tagged O-GlcNAc Transferase

Depositor

Suzanne Walker

Publication

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Tags

2x FLAG and mKate2

Mutation

WT OGT

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP

Plasmid

#157733

Purpose

mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP

Expression

Mammalian

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-Flag-hSPDEF WT

Plasmid

#100873

Purpose

Lentiviral expression of human SPDEF

Depositor

Noah Shroyer

Publication

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE2

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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RNF6_pLX307

Plasmid

#98366

Purpose

Lentiviral expression of RNF6

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RNF6 (RNF6 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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LIMD1_pLX307

Plasmid

#98349

Purpose

Lentiviral expression of LIMD1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

LIMD1 (LIMD1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Publication

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BRAC

Expression

Mammalian

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pQTEV-MAT2B

Plasmid

#34795

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

methionine adenosyltransferase II, beta (MAT2B Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-TRP1

Plasmid

#41601

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

13Myc and yeast ADH1 terminator

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pQTEV-LOC57019

Plasmid

#34811

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cytokine induced apoptosis inhibitor 1 (CIAPIN1 Human)

Tags

His and TEV

Expression

Bacterial

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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NEDD4L

Plasmid

#25169

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

nedd4l.0574.0947 (NEDD4L Human)

Tags

His

Expression

Bacterial

Mutation

Hect Domain

Available since

Aug. 9, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
pDONR223-PIK3CB

Plasmid

#23542

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3CB (PIK3CB Human)

Use

Gateway Cloning

Mutation

I6L

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pPyCAG-Myc-ERas-IP

Plasmid

#15690

Depositor

Shinya Yamanaka

Publication

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

ERas (Eras Mouse)

Tags

Myc (6x)

Expression

Mammalian

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only