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pMC7-XI3dw

Plasmid

#176171

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-3

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI5up

Plasmid

#176172

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-5

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI5dw

Plasmid

#176173

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-5

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XII5up

Plasmid

#176176

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XII-5

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XII5dw

Plasmid

#176177

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XII-5

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-X2up

Plasmid

#176162

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site X-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-X2dw

Plasmid

#176163

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site X-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Pr2-T7RNAP

Plasmid

#67739

Purpose

OR2-OR1-P2 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr2

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoBp-GFPuv

Plasmid

#139073

Purpose

Low phosphate inducible promoter phoBp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoBp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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BC-A1-014

Plasmid

#78702

Purpose

A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-SNAP-HCA_sensor

Plasmid

#127181

Purpose

Bacterial expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Tags

HisTag and StrepTag

Expression

Bacterial

Promoter

T7

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-nirButterfly

Plasmid

#136590

Purpose

Expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Article

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Insert

nirButterfly

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-ugpBp-GFPuv

Plasmid

#139078

Purpose

Low phosphate inducible promoter ugpBp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

ugpBp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-SwiChR-eYFP

Plasmid

#166604

Purpose

Encodes Cre-dependent SwiChR-eYFP under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

SwiChR-eYFP

Use

AAV

Promoter

tetracycline response element

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Pr2-deGFP-MGapt

Plasmid

#67736

Purpose

OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr2

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Pr1-deGFP-MGapt

Plasmid

#67735

Purpose

OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr1

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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DVA_AG

Plasmid

#66042

Purpose

MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS6_4323_v1

Plasmid

#126859

Purpose

4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS6_4323_v1

Use

Synthetic Biology

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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S3_004_026_MV1

Plasmid

#134416

Purpose

pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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