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pABY-c45

Plasmid

#201771

Purpose

Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-IQGAP1

Plasmid

#30112

Depositor

David Sacks

Article

Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

Insert

IQGAP1 (IQGAP1 Human)

Tags

tandem EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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HSA3_His

Plasmid

#202765

Purpose

human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA3

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEF mito-cGFP (matrix)

Plasmid

#188904

Purpose

Expresses mitochondrial GFP

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial GFP

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-IQGAP1

Plasmid

#30118

Depositor

David Sacks

Article

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG LOC550643 pFCPGW

Plasmid

#86853

Purpose

retroviral expression of NoBody

Depositor

Sarah Slavoff

Article

D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

Insert

LOC550643 (NBDY )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pABY-c25

Plasmid

#201769

Purpose

Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

CYC1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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HSA1_His

Plasmid

#202763

Purpose

human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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BSA1_His

Plasmid

#202766

Purpose

bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-c15

Plasmid

#201768

Purpose

Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSP6-EBNA(2A+DBD)

Plasmid

#98749

Purpose

In vitro transcription of modified EBNA1 mRNA

Depositor

Sara Howden, Jim Vadolas

Article

Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

Insert

Modified EBNA1

Use

In vitro transcription

Promoter

SP6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_16

Plasmid

#102292

Purpose

Yeast Surface Display of 5Ins_16 scFV

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Insulin binding scFV

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pABY-c46

Plasmid

#201774

Purpose

Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_EV3

Plasmid

#102293

Purpose

Yeast Surface Display of 5Ins_16_EV3

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Evolved Insulin binding scFV

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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BSA2_His

Plasmid

#202767

Purpose

bovine serum albumin design BSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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BSA3_His

Plasmid

#202768

Purpose

bovine serum albumin design BSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHelper_O

Plasmid

#173783

Purpose

The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

186 Integrase

Use

Synthetic Biology

Expression

Bacterial

Mutation

E59G - please see depositor comments

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_W

Plasmid

#173784

Purpose

The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Wphi Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_D

Plasmid

#173781

Purpose

The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

D145 Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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