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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,808 results for: T7

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Showing: 8281 - 8300 of 10808 results

  1. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Article
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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  4. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid
    #107292
    Purpose
    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Dmytro Yushchenko
    Article
    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191
    Insert
    SNCA-GFP (SNCA Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107292-plasmid-map-sequence-id-209472

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  13. FFA2-DuET

    Plasmid
    #213238
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FFA2 (FFAR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213238-plasmid-map-sequence-id-430796

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  17. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα13-LgB106 (GNA13 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

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Showing: 8281 - 8300 of 10808 results