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pMALB

Plasmid

#161784

Purpose

Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and hPGK

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIVT/Smarca4

Plasmid

#122146

Purpose

For making Smarca4 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarca4 (Smarca4 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP RHIM mut

Plasmid

#41384

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP(long)-FLEX-GCaMP7f

Plasmid

#189612

Purpose

Cre-dependent GCaMP7f under the GFAP promoter

Depositor

Martin Riccomagno

Article

Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

Insert

GCaMP7f

Use

AAV and Cre/Lox

Tags

GCaMP7f

Expression

Mammalian

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

Plasmid

#62952

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMW357

Plasmid

#238204

Purpose

Expresses untagged TaTasR from a PBAD promoter, followed by the native TIGR array (8 units)

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR223-BMRF1(EA-D)

Plasmid

#195807

Purpose

Gateway Entry Clone of Epstein-Barr virus BMRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BMRF1 (BMRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-TEX264 LIR4A-FLAG

Plasmid

#128259

Purpose

Expresses TEX264 LIR4A in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

TEX264 LIR4A (TEX264 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed FEEL (aa 273-276) to alanines

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-UBE3B-HA-IRES-Puro

Plasmid

#107346

Purpose

Expresses HA tagged UBE3B in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCEP4-BaLgp140-TM

Plasmid

#111843

Purpose

Mammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tether

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and TM helix from MHC class I

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

Plasmid

#62953

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
pENTR223-BZLF2

Plasmid

#195784

Purpose

Gateway Entry Clone of Epstein-Barr virus BZLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BZLF2 (BZLF2 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-EVE-MM

Plasmid

#22994

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A, S463E, S465E

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pETM33_PDCD6IP_ALIX V-domain

Plasmid

#178483

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PDCD6IP_ALIX V-domain (PDCD6IP Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_085

Plasmid

#158583

Purpose

Lentiviral expression of SpCas9; P2A site provides mKate2

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

SpCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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IL2 pAcGP67a

Plasmid

#41806

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

IL2 (IL2 Human)

Tags

6x His

Expression

Insect

Promoter

Polyhedron

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 GRK2 S670D

Plasmid

#45756

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 S670D (GRK2 Bovine)

Expression

Mammalian

Mutation

S670D

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-Akt(K179M)

Plasmid

#39535

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(K179M) (Akt1 Mouse)

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

K179M

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
REEP6-EGFP

Plasmid

#210364

Purpose

Expresses REEP6 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP6 (REEP6 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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