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pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Plasmid

#159801

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

Plasmid

#191177

Purpose

Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (frameshifted)

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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enAsABE8e-dimer

Plasmid

#138507

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTW080

Plasmid

#115928

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/30 SGP NA (No Aptamer)

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T2-Snf1-cat

Plasmid

#52683

Purpose

expression of the Snf1 kinase domain in bacteria

Depositor

Benjamin Turk

Article

Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

Insert

SNF1 (SNF1 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

encodes kinase domain only, aa 41-315

Promoter

tac

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pMKO.1-shPrc1

Plasmid

#160959

Purpose

Prc1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Prc1 shRNA (Prc1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 S199A

Plasmid

#78806

Purpose

To overexpress RIPK3 S199A in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

S199A

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Ago3m

Plasmid

#21531

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago3 (AGO3 Human)

Tags

GFP

Expression

Mammalian

Mutation

missing aa757-823, the C-termianl 66aa of PIWI do…

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only