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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,399 results for: sacs

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Showing: 8341 - 8360 of 9399 results

  1. pDC10

    Plasmid
    #75077
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Insert
    phosphoribosylglycinamide synthetase (purD Escherichia coli)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    adds unique SacII site at Thr95-Ala96 by a silent…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    75077-plasmid-map-sequence-id-142310

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  2. pZP06C

    Plasmid
    #232322
    Purpose
    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.
    Depositor
    Chenggang Wu
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    rpsJ
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232322-plasmid-map-sequence-id-462278

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  3. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114274-plasmid-map-sequence-id-218322

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  6. pHelper_NoMutL_V2_gentR

    Plasmid
    #205293
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    205293-plasmid-map-sequence-id-410127

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  7. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid
    #169389
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP16
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169389-plasmid-map-sequence-id-336165

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  8. pHelper_Ec1_V1_gentR

    Plasmid
    #205291
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205291-plasmid-map-sequence-id-405409

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  9. pHelper_Ec1_V1_ampR

    Plasmid
    #205292
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205292-plasmid-map-sequence-id-404792

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  10. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Promoter
    polyhedrin
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

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  11. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169384-plasmid-map-sequence-id-336496

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  12. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169391-plasmid-map-sequence-id-336874

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  13. pmCellFree_KA1_SARS-COV-2 _NSP1

    Plasmid
    #169376
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP1
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169376-plasmid-map-sequence-id-335277

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  14. pHH0103 WWOX WW domain #1

    Plasmid
    #104235
    Purpose
    Bacterial expression of WW domain #1 from WWOX
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWOX WW domain #1 (WWOX Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104235-plasmid-map-sequence-id-204370

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  15. pTU1-D-spsCD

    Plasmid
    #112788
    Purpose
    Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    spsCD
    Expression
    Bacterial
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112788-plasmid-map-sequence-id-218383

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  16. pTU1-A-spsCU

    Plasmid
    #112787
    Purpose
    Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    spsCU
    Expression
    Bacterial
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112787-plasmid-map-sequence-id-218381

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  17. pHH0103 WWOX WW domain #2

    Plasmid
    #104236
    Purpose
    Bacterial expression of WW domain #2 from WWOX
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWOX WW domain #2 (WWOX Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104236-plasmid-map-sequence-id-204371

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  18. pMU131

    Plasmid
    #66811
    Purpose
    Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI
    Depositor
    Lee Lynd
    Article
    Shaw et al Appl Environ Microbiol. 2010 Jul;76(14):
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66811-plasmid-map-sequence-id-117172

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  20. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid
    #169390
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169390-plasmid-map-sequence-id-336867

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Showing: 8341 - 8360 of 9399 results