Addgene

We narrowed to 19,618 results for: REN;

Showing: 2051 - 2100 of 19618 results

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pBSV2_Psyn-sgRNA500

Plasmid

#149613

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP18-tetR_O

Plasmid

#127021

Purpose

Basic Part O- ORF; TetR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part TetR_o

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_BGL2

Plasmid

#101911

Purpose

Expresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.

Depositor

Steve Shih

Article

Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

Insert

β-glucosidase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7+LacO

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 PCNA GFP

Plasmid

#105937

Purpose

in vitro transcription, cell cycle nuclear marker

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

PCNA (PCNA Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-L26-Flag

Plasmid

#19972

Depositor

Moshe Oren

Article

Ofir-Rosenfeld et al Mol Cell. 2008 Oct 24. 32(2):180-9.

Insert

Rpl26 (RPL26 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only
pJUMP44-2A(sfGFP)

Plasmid

#126988

Purpose

Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC4M-F2E-GFP-FKBP

Plasmid

#68058

Purpose

express EGFP-tagged FKBP for heterodimerization with FRB in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

EGFP

Use

Tags

2xFKBP and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pJUMP24-1A(sfGFP)

Plasmid

#126971

Purpose

Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MyoD-pCLBabe

Plasmid

#20917

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

MyoD (Myod1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pFLAG-Cherry-N1-PRKAG1

Plasmid

#30308

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase γ1 (PRKAA1 Human)

Use

Tags

FLAG and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
pMito.iGABASnFR.F102Y.Y137L

Plasmid

#112177

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

Tags

Expression

Mammalian

Mutation

F102Y.Y137L

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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MSGV Hu Acceptor PGK-NGFR

Plasmid

#64270

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Use

Tags

T7 tag and T7RNAP

Expression

Bacterial

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PRKAA1ΔC

Plasmid

#30306

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 ΔC (Prkaa1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids Pro 526 to Gln 548

Promoter

Available since

Aug. 26, 2011

Availability

Academic Institutions and Nonprofits only
TOPO mTagBFP2

Plasmid

#68413

Purpose

TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mTagBFP2

Use

Gmap

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMito.iGABASnFR.F102G

Plasmid

#112176

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

Tags

Expression

Mammalian

Mutation

F102G

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Nup88

Plasmid

#64281

Purpose

Mammalian expression of full-length Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only
pESC/mCitrine

Plasmid

#117996

Purpose

Expression of mCitrine controlled by the Gal1 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

mCitrine

Use

Yeast/bacteria binary vector

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-merR_O

Plasmid

#127023

Purpose

Basic Part O- ORF; merR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part merR_o

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
R26TV CAG LSL 2-5

Plasmid

#68412

Purpose

Rosa26 targeting vector backbone for use with GMAP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting; Gmap

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO CFP

Plasmid

#68489

Purpose

TOPO construct expressing CFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cyan Fluorescent Protein

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA231-CRISPR

Plasmid

#138712

Purpose

Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Tags

Expression

Plant

Mutation

D156R

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO mCherry

Plasmid

#68490

Purpose

TOPO construct expressing mCherry for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mCherry

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMito.iGABASnFR.F102G.mRuby3

Plasmid

#112180

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

Tags

mRuby3

Expression

Mammalian

Mutation

F102G

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pB_35S/mEGFP

Plasmid

#135320

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP42x-2A(sfGFP)

Plasmid

#126986

Purpose

Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PAX6 TALEN R

Plasmid

#105525

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

Pax6 TALEN R

Use

TALEN

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PAX6 TALEN L

Plasmid

#109034

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

Pax6 TALEN L

Use

TALEN

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO PuroR

Plasmid

#68447

Purpose

TOPO construct expressing PuroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO ZeoR

Plasmid

#68446

Purpose

TOPO construct expressing ZeoR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Zeocin Resistance

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO HygroR

Plasmid

#68445

Purpose

TOPO construct expressing HygroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Hygromycin Resistance

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FFiBlast p5

Plasmid

#68470

Purpose

Inverted and floxed blasticidin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9S

Plasmid

#149638

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, empty

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-cueR_O

Plasmid

#127026

Purpose

Basic Part O- ORF; cueR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part cueR_o

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO EFS

Plasmid

#68451

Purpose

TOPO construct expressing EFS for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Elongation Factor 1α Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P2_crU626

Plasmid

#191777

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P7_crU626

Plasmid

#191782

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221TrxA-AID-T7RNAP

Plasmid

#167976

Purpose

Expresses fusion protein TrxA-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TrxA-AID-T7RNAP

Use

Tags

T7RNAP and TrxA

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP22x-1A(sfGFP)

Plasmid

#126969

Purpose

Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TOPO iRFP670

Plasmid

#68443

Purpose

TOPO construct expressing iRFP670 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

iRFP670

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CD3delta-CFP

Plasmid

#128154

Purpose

Expression of CD3 delta

Depositor

Jennifer Lippincott-Schwartz

Article

Lorenz et al Nat Methods. 2006 Mar;3(3):205-10. doi:

Insert

CD3 Delta (Cd3d Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHNFI-B2

Plasmid

#31405

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IB isoform 2 (Nfib Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Not the same as reference seq. See sequence for m…

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P3_crU626

Plasmid

#191778

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P4_crU626

Plasmid

#191779

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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