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Plasmid#203838PurposeFlp-dependent Cre and mScarlet-KASH expressionDepositorInsertCre
UseAAVTagsmScarlet-KASH-2AExpressionMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-ChRmine-oScarlet
Plasmid#137159PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
iF66 PB-LoxStopLox-Zim3-dCas9-mScarlet-hygro
Plasmid#204731PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-oScarlet
Plasmid#137137PurposeIntersectional viral expression of oScarlet in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre
Plasmid#203839PurposeFlp-dependent enzymatically inactive Cre and mScarlet-KASH expressionDepositorInsertdCre
UseAAVTagsmScarlet-KASH-2AExpressionMutationEnzymatically inactive CrePromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-4-IRES-mScarlet
Plasmid#209410PurposeExpression of wild-type neonatal isoform of human SCN2A.DepositorInsertSCN2A Variant 4, stabilized (SCN2A Human)
UseTagsExpressionMammalianMutationIVS intron inserted after bp 4551 of the SCN2A OR…PromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1
Plasmid#214994PurposeExpress the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.DepositorInsertEEA1 (EEA1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE
Plasmid#125713PurposeCre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarletDepositorHas ServiceAAV1 and AAV5InserteOPN3
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationdeleted amino acids 331-429PromoterhSyn1Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE
Plasmid#130991PurposeAAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoterDepositorHas ServiceAAV8InsertChRmine
UseAAVTagsmScarlet-Kv2.1ExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183519PurposeOptogeneticsDepositorHas ServiceAAV8InsertChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)
Plasmid#224536PurposeA Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3DepositorInsertIRES-FLAG- NLS-mScarlet-I3
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChRmine-oScarlet
Plasmid#137160PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
iF65 PB-FrtStopFrt-Zim3-dCas9-mScarlet-hygro
Plasmid#204730PurposeFrt-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE
Plasmid#198508PurposeExpresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.DepositorInsertPdCO
UseAAVTagsRho1D4 and mScarletExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-mScarlet-I-P2A-iCre (JDW 917)
Plasmid#224516PurposeA Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)DepositorInsertmScarlet-I-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3
Plasmid#111925PurposeCyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μDepositorInsertCeNL(Ca2+)_110μ
UseTagsCalreticulin and KDELExpressionMammalianMutationE31D, F92W, E104D. D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hsChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183521PurposeOptogeneticsDepositorInserthsChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationH33RPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-SIO-PdCO-mScarlet-WPRE
Plasmid#198509PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.DepositorInsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette
Plasmid#169737PurposeEqualizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-mScarlet-MCS-polyA
Plasmid#174023PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertmScarlet
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only