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Plasmid#86298PurposeEncodes gRNA for 3' target of human KDM1ADepositorInsertgRNA against KDM1A (KDM1A Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1871
Plasmid#205976PurposeU6-JAGBLH010000220 Cas8-HNH DR-BpiI-DRDepositorInsertCas8-HNH DR-BpiI-DR
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPS0382
Plasmid#133232PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2
Plasmid#112004PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mKate2DepositorHas ServiceAAV9Insertaxon-GCaMP6s-P2A-mKate2
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS1543
Plasmid#205963PurposeNZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)DepositorInsertDinG HNH Cascade with His14-tagged Csf3
UseTags14xHis on Csf3ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1517
Plasmid#205980PurposeFLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_MIER3
Plasmid#86274PurposeDonor vector for 3' FLAG tag of human MIER3DepositorInsertMIER3 homology arms (MIER3 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_CEBPA
Plasmid#86293PurposeDonor vector for 3' FLAG tag of human CEBPADepositorInsertCEBPA homology arms (CEBPA Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_AHR_2
Plasmid#101077PurposeEncodes gRNA for 3' target of human AHRDepositorInsertgRNA against AHR (AHR Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1929
Plasmid#205968PurposeJAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1DepositorInsertCas8-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ARID5B
Plasmid#86288PurposeDonor vector for 3' FLAG tag of human ARID5BDepositorInsertARID5B homology arms (ARID5B Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1516
Plasmid#205979PurposeFLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas7 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1477
Plasmid#205970PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)DepositorInsertCas5-HNH all proteins, with His-tagged Cas11
UseTags14xHis on Cas11ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover
Plasmid#124549PurposeLentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)DepositorInsertp65 promoter- p65 CDS-Clover
UseLentiviralTagsCloverExpressionMammalianMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS1068
Plasmid#205962PurposeNZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promotersDepositorInsertDinG HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458_KDM6A_1
Plasmid#86307PurposeEncodes gRNA for 3' target of human KDM6ADepositorInsertgRNA against KDM6A (KDM6A Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_FOXP1
Plasmid#86261PurposeDonor vector for 3' FLAG tag of human FOXP1DepositorInsertFOXP1 homology arms (FOXP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KAT7
Plasmid#86266PurposeDonor vector for 3' FLAG tag of human KAT7DepositorInsertKAT7 homology arms (KAT7 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only