Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,500 results for: TIM

Clear keywords
Showing: 821 - 840 of 14500 results

  1. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV1)

    Viral Prep
    #125712-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Promoter
    short version of Ī±CamKinase II promoter (0.4kb)
    Tags
    mScarlet
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  2. pAAV_hSyn-PdCO-EGFP-WPRE (AAV5)

    Viral Prep
    #198513-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  3. pAAV_hSyn-PdCO-EGFP-WPRE (AAV1)

    Viral Prep
    #198513-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV_hSyn-PdCO-mScarlet-WPRE (AAV5)

    Viral Prep
    #198510-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn-PdCO-mScarlet-WPRE (#198510). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV_hSyn-PdCO-mScarlet-WPRE (AAV1)

    Viral Prep
    #198510-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-mScarlet-WPRE (#198510). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only

  6. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    Collection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  7. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219ā€¦
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

    Enlarge

  8. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid
    #185917
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219ā€¦
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185917-plasmid-map-sequence-id-367357

    Enlarge

  11. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV5)

    Viral Prep
    #198511-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet (Cre-dependent)
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  12. pGP-AAV-syn-FLEX-Volton2-ST-WPRE (AAV1)

    Viral Prep
    #172907-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-Volton2-ST-WPRE (#172907). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-Volton2-ST-WPRE plasmid DNA. Cre-dependent, Syn-driven expression of the voltage sensor Voltron2-ST containing a soma localization targeting sequence. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Promoter
    Syn
    Available Since
    July 21, 2023
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV1)

    Viral Prep
    #198511-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet (Cre-dependent)
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV5)

    Viral Prep
    #198516-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    EF-1a
    Tags
    mScarlet
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV Retrograde)

    Viral Prep
    #125712-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Promoter
    short version of Ī±CamKinase II promoter (0.4kb)
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV1)

    Viral Prep
    #198516-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    EF-1a
    Tags
    mScarlet
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  17. pCI-TPI_WT-IL6_3UTR-xrRNA-4H

    Plasmid
    #108373
    Purpose
    Expresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial IL6 control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, IL6 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108373-plasmid-map-sequence-id-208350

    Enlarge

  18. pT2HE_BEPA_MYH3C2-C3

    Plasmid
    #250496
    Purpose
    2.8 kb muscle enhancer derived from freshwater Bear Paw Lake, AK stickleback driving GFP expression
    Depositor
    David Kingsley
    Article
    Yoxsimer et al Current Biology (2026)
    Insert
    BEPA_MYH3C2-C3_intergenic_region
    Use
    Gene expression and genomic integration in fish
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    250496-plasmid-map-sequence-id-499058

    Enlarge

  20. pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H

    Plasmid
    #108372
    Purpose
    Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, RAB7A Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    108372-plasmid-map-sequence-id-209438

    Enlarge

Showing: 821 - 840 of 14500 results