We narrowed to 51,185 results for: eng

1. pY3

   Plasmid

   #218116

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY3-LYS-IV

   Plasmid

   #218119

   Purpose

   LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNVLTv2-pvdIJD-Ptrc-base

   Plasmid

   #169243

   Purpose

   Suicide vector for integrating expression cassette in P. putida (IPTG induction)

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   lacI
   

   Expression

   Bacterial

   Promoter

   lacIq

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-Casp2

   Plasmid

   #50023

   Purpose

   pENTR vector for mouse Caspase-2

   Depositor

   Jamie Davies

   Article

   Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

   Insert

   Casp2 (Casp2 Mouse)
   

   Use

   Entry vector

   Available Since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCORE-Hp53

   Plasmid

   #72242

   Purpose

   Contains the CORE cassette with hyg and human p53 gene markers

   Depositor

   Michael Resnick, Francesca Storici

   Article

   Storici et al Genet Eng (N Y). 2003;25:189-207.

   Inserts

   p53 (TP53 Human)
   hyg
   

   Expression

   Bacterial

   Mutation

   V122A mutation

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCTCON2-GFP-TAG

   Plasmid

   #158131

   Purpose

   Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   sfGFP
   

   Expression

   Yeast

   Mutation

   Introduced a stop codon in place of the tyrosine …

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas5

   Plasmid

   #82378

   Purpose

   sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23119

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFOX6-Tier1-PhCMV-TdTomato

   Plasmid

   #169515

   Purpose

   Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven dimeric red fluorescent protein TdTomato
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH613

   Plasmid

   #169602

   Purpose

   Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4 / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Expression

    Yeast

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbE1k-2

    Plasmid

    #59945

    Purpose

    dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase
    pterin-4a-carbinolamine dehydratase
    

    Promoter

    pTrc

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas2F

    Plasmid

    #82377

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pChlr-7SE

    Plasmid

    #52168

    Purpose

    Encodes module 7 with amino acids 12S-16E for recognition of nt 2G

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM545-Tier1-PhCMV-Citrine

    Plasmid

    #169505

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven yellow fluorescent protein citrine
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB303-Tier1-PhCMV-TetR_VPR

    Plasmid

    #169575

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled transactivator
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAB301-Tier1-PhCMV-rTetR_VPR

    Plasmid

    #169579

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    reverse tetracycline-controlled transactivator
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVH227-Tier1-OTetO7-PCMVmin2-SEAP

    Plasmid

    #169563

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP production
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFOX13-Tier1-PhCMV-tGFP

    Plasmid

    #169510

    Purpose

    Tier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven turbo green fluorescent protein
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pChlr-4SYE

    Plasmid

    #52156

    Purpose

    Encodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    D43951 (PUM1 Human)
    

    Expression

    Bacterial

    Mutation

    Changed 12N to 12S, 13H to 13Y, and 16Q to 16E in…

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only