We narrowed to 2,798 results for: fas

1. pAB120

   Plasmid

   #167150

   Purpose

   MoClo-compatible Level 1 (position 1) vector encoding Kanamycin resistance cassette, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   pNOS-nptII-ocsT
   

   Expression

   Plant

   Promoter

   pNOS

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX022

   Plasmid

   #167148

   Purpose

   MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   C-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX021

   Plasmid

   #167147

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   N-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCS-mGold2t

   Plasmid

   #231766

   Purpose

   Expression of mGold2t in bacterial cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t
   

   Expression

   Bacterial

   Mutation

   mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   Synthetic promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCaggs-mGold2t

   Plasmid

   #231764

   Purpose

   Expression of mGold2t in mammalian cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t
   

   Expression

   Mammalian

   Mutation

   mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmEos2-N1 GBP (GBP-mEos2)

   Plasmid

   #162876

   Purpose

   Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   GFP Binding Protein tagged with mEos2
   

   Tags

   mEos2

   Expression

   Mammalian

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHybr_r2a>mA-srt-his

   Plasmid

   #124806

   Purpose

   HDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgA
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CIRTS-control

   Plasmid

   #213788

   Purpose

   CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

   Insert

   CIRTS-Calm3-U6-control gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn1, U6

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CIRTS-Gas5

   Plasmid

   #213172

   Purpose

   CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

   Insert

   CIRTS-Calm3-U6-Gas5 gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn1, U6

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHybr_r2a>mG2b-srt-his

    Plasmid

    #124804

    Purpose

    HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG2b
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHybr_r2a>mG2a-srt-his

    Plasmid

    #124803

    Purpose

    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG2a
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX459-gR2A_Hinge

    Plasmid

    #124811

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Cas9 – gRNA_R2A_hinge
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    R166H in PuroR (please see depositors comment bel…

    Promoter

    pUC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHybr_r2a>mG1-srt-his

    Plasmid

    #124802

    Purpose

    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG1
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Promoter

    -

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHybr_r2a>mG3-srt-his

    Plasmid

    #124805

    Purpose

    HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG3
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL-mGold2t

    Plasmid

    #231762

    Purpose

    Expression of mGold2t in yeast cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Expression

    Yeast

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    pTDH(GAP promoter)

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPF101-pfQC-cyclase dead

    Plasmid

    #184878

    Purpose

    expresses soluble plasmodium falciparum QC protein with a histag, cyclase dead mutant

    Depositor

    Ferenc Scheeren

    Article

    Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    pfQC-cyclase dead
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    F77A & Q79A

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBlue-hFX(I154F)-HA

    Plasmid

    #14988

    Depositor

    Michael Ristow

    Insert

    human frataxin cDNA (FXN Human)
    

    Tags

    AU1 and HA

    Expression

    Bacterial

    Mutation

    changed isoleucine 154 to phenylalanine

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pBlue-hFX(T133A)-HA

    Plasmid

    #14989

    Depositor

    Michael Ristow

    Insert

    human frataxin cDNA (FXN Human)
    

    Tags

    AU1 and HA

    Expression

    Bacterial

    Mutation

    changed threonine 133 to alanine

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pBlue-hFX(T133E)-HA

    Plasmid

    #14990

    Depositor

    Michael Ristow

    Insert

    human frataxin cDNA (FXN Human)
    

    Tags

    AU1 and HA

    Expression

    Bacterial

    Mutation

    changed threonine 133 to glutamic acid

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pBlue-hFX-HA(AscI)

    Plasmid

    #15404

    Depositor

    Michael Ristow

    Insert

    human frataxin cDNA (FXN Human)
    

    Tags

    AU1 and HA

    Expression

    Bacterial

    Mutation

    700 bp insert can be released by digestion with r…

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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