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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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PJ-INPP5E

Plasmid

#38001

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1 (SAC1 Budding Yeast)
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Mutation

Cys 779 mutated to Ser

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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GBX

Plasmid

#64123

Purpose

a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

GFP-2A-BCL2L1 (BCL2 Human)

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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iRFP-FRB-Rab5

Plasmid

#51612

Purpose

Recruiter to Rab5-positive endosomes

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FRB
Rab5

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST p38KTRmCerulean3

Plasmid

#59155

Purpose

Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ131A

Plasmid

#69273

Purpose

Golden Gate entry vector; 1st gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBS mNotch1-IC (DM#270)

Plasmid

#15079

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

Notch1-IC (Notch1 Mouse)

Expression

Bacterial

Mutation

K1750A

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pTPGI_dCas9_VP64

Plasmid

#49013

Purpose

encodes yeast-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (codon-optimized for expression in S. cerevisiae)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Yeast

Promoter

pTPGI (galactose+aTc inducible)

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-PE2max-BSD

Plasmid

#191102

Purpose

Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

PEmax-P2A-BSD

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgG4/κ

Plasmid

#61887

Purpose

Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-PR rep/cap

Plasmid

#197565

Purpose

encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

Depositor

Casey Maguire

Article

Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

Inserts

AAV Rep genes
AAV9 VP1 Cap gene with PR insert

Use

AAV

Mutation

contains 7mer insert PRPPSTH between amino acids …

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

Plasmid

#31358

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

JMJD6 (JMJD6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Expression

Mammalian

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-H2BtdiRFP-IP

Plasmid

#47884

Purpose

Histone H2B fused with tandem iRFP, ires-puro

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

H2B-tdiRFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

Plasmid

#163047

Purpose

GCaMPer calcium sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMPer

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-4

Plasmid

#72679

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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