We narrowed to 905 results for: pgem

1. C078

   Plasmid

   #174347

   Purpose

   Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   ePAB
   

   Use

   In vitro transcription template

   Tags

   FLAG

   Mutation

   13 silent mutations: G243A, A273G, G339A, C345G, …

   Availability

   Academic Institutions and Nonprofits only

   

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2. C233

   Plasmid

   #174349

   Purpose

   Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   PABPC1 (M161A) (pabpc1.S Frog)
   

   Use

   In vitro transcription template

   Tags

   FLAG

   Mutation

   M161A

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGKpuro

   Plasmid

   #11349

   Depositor

   Rudolf Jaenisch

   Article

   Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

   Insert

   pgk-1 5' and 3' regions (Pgk1 Mouse)
   

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSFV-Helper1

   Plasmid

   #92073

   Purpose

   This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

   Depositor

   Henrik Garoff

   Article

   Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

   Insert

   cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
   

   Use

   Helper plasmid, for run-off transcription and rna…

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1.3xWT HBV-Luciferase

   Plasmid

   #61549

   Purpose

   The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core

   Depositor

   Yosef Shaul

   Article

   Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.

   Insert

   1.3 times of the HBV genome with luciferase within the Core ORF
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Substitution of HBV sequences with luciferase ORF…

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTnos (GB0037)

   Plasmid

   #68188

   Purpose

   Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Nos terminator
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pP35S (GB0030)

   Plasmid

   #68163

   Purpose

   Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   CaMV 35S promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGUS (GB0208)

   Plasmid

   #68203

   Purpose

   Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   GUS coding region
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_hMed25

   Plasmid

   #206958

   Purpose

   The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

   Depositor

   Reza Zolfaghari, Catharine Ross

   Insert

   Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBFP (GB0025)

    Plasmid

    #68193

    Purpose

    Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    BFP coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPnos (GB0072)

    Plasmid

    #68166

    Purpose

    Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Nos promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDsRed (GB0100)

    Plasmid

    #68202

    Purpose

    Provides the CDS of DsRed as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    DsRed coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. 7iBi89 (rat fibronectin)

    Plasmid

    #14065

    Depositor

    Richard Hynes

    Article

    Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

    Insert

    Rat Fibronectin 7iBi89 (Fn1 Rat)
    

    Expression

    Mammalian

    Mutation

    Insert was cut out of pGEM3 with SalI and SacI an…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pYFP (GB0053)

    Plasmid

    #68197

    Purpose

    Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    YFP coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPromE8 (GB0914)

    Plasmid

    #68175

    Purpose

    Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlE8 promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT35S (GB0036)

    Plasmid

    #68187

    Purpose

    Provides the CaMV 35S terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV 35S terminator
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pP2A11 (GB0082)

    Plasmid

    #68168

    Purpose

    Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Sl2A11 promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. BtA2-3A

    Plasmid

    #238984

    Purpose

    In vitro transcription of mutant bovine arrestin-2 long isoform

    Depositor

    Vsevolod Gurevich

    Article

    Celver et al J Biol Chem. 2002 Mar 15;277(11):9043-8.

    Insert

    arrestin-2 long (ARRB2 Bovine)
    

    Use

    In vitro transcription

    Mutation

    I386A,V387A,F388A

    Promoter

    SP6

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. BtA1N

    Plasmid

    #238979

    Purpose

    In vitro transcription of bovine arrestin-1

    Depositor

    Vsevolod Gurevich

    Article

    Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

    Insert

    arrestin-1 (SAG Bovine)
    

    Use

    In vitro transcription

    Mutation

    silent

    Promoter

    SP6

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. BtA2N

    Plasmid

    #238980

    Purpose

    In vitro transcription of bovine arrestin-2 long isoform

    Depositor

    Vsevolod Gurevich

    Article

    Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

    Insert

    arrestin-2 long (ARRB2 Bovine)
    

    Use

    In vitro transcription

    Mutation

    silent

    Promoter

    SP6

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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