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pRC01

Plasmid

#114680

Purpose

sfGFP.Strands 1-9

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP Strands 1-9

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLG_GI3

Plasmid

#111594

Purpose

hU6-sgRNA GI Library vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-calx1

Plasmid

#58697

Purpose

Expression of calx1

Depositor

Craig Montell

Article

Wang et al Neuron 2005 Feb 3;45(3):367-78.

Insert

calx1 (Calx Fly)

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
Neg. Ctrl_sgRNA

Plasmid

#111298

Purpose

CRISPR KO murine neg. ctrl.

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

NegCtrl sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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MsCLYBL_sgRNA1

Plasmid

#111294

Purpose

CRISPR KO murine CLYBL

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine CLYBL

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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CaYin1

Plasmid

#33067

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1

Tags

6 His tag

Expression

Bacterial

Promoter

ARA promoter

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pH6Z-806

Plasmid

#85255

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MyD88-Rip3

Plasmid

#131352

Purpose

Expresses MyD88 with Rip3 fused to its C-terminus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-Rip3 (Myd88 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Banshee-Sh-Ctrl

Plasmid

#85587

Purpose

Control hairpin sequence used in gene knockdown experiements

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

sh-Ctrl

Use

Retroviral

Expression

Mammalian

Promoter

H1

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-17

Plasmid

#119996

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR Age HsSAS-6 ST

Plasmid

#46382

Purpose

For expression of N-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Gateway Cloning

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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YN2_1_IL50_HOlocus

Plasmid

#194426

Purpose

Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

Cas9

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MsCLYBL_sgRNA6

Plasmid

#111295

Purpose

CRISPR KO murine CLYBL

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA6 against murine CLYBL

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pL_FR_Hygro

Plasmid

#127171

Purpose

Frameshift reporter (control plasmid for screening)

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

Frameshift Reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCG-A-sacB(SP)-B

Plasmid

#87370

Purpose

An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SacB signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-27

Plasmid

#120006

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC572

Plasmid

#62318

Purpose

sgRNA with 1x com for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x com RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCG-D-SpyTag-His6-E

Plasmid

#87375

Purpose

An ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SpyTag

Use

Synthetic Biology

Tags

His6 tag

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-SP-FKBP-mCherry-TM-V5

Plasmid

#186226

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

SP-FKBP-mCherry-TM-V5

Tags

V5

Expression

Mammalian

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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