166,542 results

1. pIRE4-Bpa

   Plasmid

   #155342

   Purpose

   Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

   Depositor

   Irene Coin

   Article

   Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

   Insert

   humanized EBpaRS/BstYam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/U6

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcGFP-N1_CBP

   Plasmid

   #221903

   Purpose

   Transient expression of GFP-tagged CBP in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBP (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-TRIM28

   Plasmid

   #124960

   Purpose

   Mammalian expression of Human TRIM28

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-NSP4SA11

   Plasmid

   #89171

   Purpose

   Express Rotavirus SA11 NSP4 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPR v2 hBAK

   Plasmid

   #129579

   Purpose

   CRISPR deletion of human BAK

   Depositor

   Stephen Tait

   Article

   Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

   Insert

   gRNA vs human BAK (BAK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJR101

   Plasmid

   #187241

   Purpose

   Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-VP2SA11

   Plasmid

   #89163

   Purpose

   Express Rotavirus SA11 VP2 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mem-TurboID

   Plasmid

   #149409

   Purpose

   Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Insert

   mem-TurboID
   

   Use

   Tags

   HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Ftractin-mCherry

    Plasmid

    #85131

    Purpose

    lentiviral expression of an F-actin live-cell marker (mCherry)

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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