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pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS

Plasmid

#154238

Purpose

Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC

Depositor

Arnold Driessen

Article

Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

Insert

pgndA:QfDBD-VP16AD-GFP-NLS

Use

Synthetic Biology; Fungal expression

Tags

GFP-NLS; DsRed-SKL

Expression

Bacterial

Promoter

pGndA (An11g02040 A. niger)

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.16-RBD

Plasmid

#213070

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.16-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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Slo1/BKAlpha maxi-K+ channel scFv [L6/60]

Plasmid

#199421

Purpose

Mammalian expression of Slo1/BKAlpha maxi-K+ channel scFv. Derived from hybridoma L6/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingSlo1/BKAlpha maxi-K+ channel (Rat) (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-TrpM6 [N470/22R]

Plasmid

#206596

Purpose

Mammalian Expression Plasmid of anti-TrpM6 (Human). Derived from hybridoma N470/22.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpM6 (Homo sapiens) recombinant Mouse monoclonal antibody (TRPM6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-ATAT1 [N347/42R]

Plasmid

#206558

Purpose

Mammalian Expression Plasmid of anti-ATAT1 (Human). Derived from hybridoma N347/42.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ATAT1 (Homo sapiens) recombinant Mouse monoclonal antibody (ATAT1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GluN2B/NR2B glutamate receptor [N59/36R-2b]

Plasmid

#199418

Purpose

Mammalian expression plasmid of Anti-GluN2B/NR2B glutamate receptor [N59/36R-2b] (Rat). Derived from hybridoma N59/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GluN2B/NR2B glutamate receptor (Rat) recombinant mouse monoclonal antibody (Grin2b Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

Plasmid

#195505

Purpose

T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)

Use

CRISPR; Donor vector

Tags

mRuby-3xFLAG-NLS-3xSV40-poly(A)

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mCherry-Rab8A

Plasmid

#194333

Purpose

For use in light-induced Rab8a protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Inserts

CIB1 (CIB1 Mustard Weed)
Rab8a (RAB8A Canis Lupus)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Kir6.1 K+ channel [N366/60R]

Plasmid

#188206

Purpose

Mammalian Expression Plasmid of anti-Kir6.1 K+ channel (Rat). Derived from hybridoma N366/60

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kir6.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnj8 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Homer1L/S [L113/130R]

Plasmid

#177457

Purpose

Mammalian Expression Plasmid of anti-Homer1L/S (Mouse). Derived from hybridoma L113/130.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Homer1L/S (Mus musculus) recombinant mouse monoclonal antibody (Homer1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-SynDIG4/Prrt1 [L102/45R]

Plasmid

#177450

Purpose

Mammalian Expression Plasmid of anti-SynDIG4/Prrt1 (Rat). Derived from hybridoma L102/45.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SynDIG4/Prrt1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Prrt1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Gamma2L [N452/81R]

Plasmid

#177554

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L (Human). Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Gamma2L/S [N452/73R]

Plasmid

#177553

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L/S (Human). Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L/S (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

Plasmid

#127867

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Human, Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

constructed by blunt end fusion of the 5’ 51 nucl…

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-GABARAPL1 G116A

Plasmid

#123118

Purpose

Expresses EGFP-GABARAPL1 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-beta2(616-951)-GFP Double

Plasmid

#100729

Purpose

To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform, deletion of clathrin box motif and …

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIB Flag MFF S155,172A

Plasmid

#74387

Purpose

Retroviral expression vector containing Flag tagged S155,172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Ala, Ser 172 Ala (Numbering based on MFF …

Promoter

CMV

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (S180A/S204A)

Plasmid

#17700

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S180A S204A (PTPRA Human)

Expression

Mammalian

Mutation

S180A / S204A

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
5mCUC1 pBluescript

Plasmid

#12065

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Expression

Bacterial

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only