We narrowed to 22,852 results for: TES

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pETM33_ZYMND11_coiled-coil MYND

Plasmid

#178493

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1

Plasmid

#98150

Purpose

Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1

Tags

His6-TEV

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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Hiv7CMV-Cremyc-2A-GFP

Plasmid

#117148

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Use

Lentiviral

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78307

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADflluw

Available Since

July 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBR322-bom

Plasmid

#155178

Purpose

pBICI_2_strong without bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMG

Plasmid

#138264

Purpose

Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Inserts

eGFP flanked by iCas9 sites
mCherry

Use

CRISPR

Expression

Yeast

Promoter

Tef1

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shMki67

Plasmid

#160958

Purpose

Mki67 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Mki67 (Mki67 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR

Plasmid

#48157

Purpose

Transgenically expresses firefly luciferase protein in T. cruzi

Depositor

Rick Tarleton

Article

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

luciferase

Use

Luciferase; T. cruzi expression

Promoter

T7

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRQ-Id1HA

Plasmid

#18112

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

Inhibitor of differentiation 1 (Id1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-6A-HA

Plasmid

#128103

Purpose

Express HA tagged Nxph4-6A

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pETM33_PTK2_Focal-AT

Plasmid

#178485

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PTK2_Focal-AT (PTK2 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGST-TEV-yVms1(1-417,delta(38-69))

Plasmid

#128739

Purpose

Bacterial overexpression and crystallization

Depositor

Chris Hill

Article

Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

Insert

Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

residues 1-417 with deletion of 38-69

Promoter

T7

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSK260

Plasmid

#129252

Purpose

dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35 NM yTRAP sensor reporting on mKate2

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-ATG12

Plasmid

#27049

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
pWB-MF-IKKe

Plasmid

#44116

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

IKKe (IKBKE Human)

Use

Retroviral

Tags

Myristoylated Flad

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway destination vector

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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deltaPYD-NLRP11.MYC

Plasmid

#131197

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

deltaPYD-NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

deletion of the pyrin domain (amino acids 1-124 m…

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRB

Plasmid

#193568

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/3xmyc_NLRP11-LRR

Plasmid

#131200

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

LRR of NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

contains only the leucine-rich repeats (LRRs), am…

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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