We narrowed to 16,204 results for: grna

1. pDECKO-mCherry MALAT1_Enhancer.1

   Plasmid

   #78536

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT-9208

   Plasmid

   #124221

   Purpose

   Cloning plasmid for a self-targeting gRNA library

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CRISPR sgSHMT1_1

   Plasmid

   #106311

   Purpose

   Express Cas9 and sgRNA targeting SHMT1

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting SHMT1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

   Plasmid

   #84260

   Purpose

   Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgSV40
   PYL1-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Promoter

   CMV and mouse U6

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_25G

   Plasmid

   #91144

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. HSF1 KO

   Plasmid

   #200207

   Purpose

   gRNA for HSF1 KO

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   HSF1 KO (Hsf1 Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDECKO-mCherry MALAT1_Exon.1

   Plasmid

   #78540

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. BII-gR-PtW-eSpCas9

   Plasmid

   #133357

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   HA-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-rev_U6_MCS

   Plasmid

   #109769

   Purpose

   Multisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequence

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   U6-gRNA cassette
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1-puro-sgULK1-1

    Plasmid

    #109004

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ULK1-sgRNA #1 (ULK1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CRISPR V2 sgMTFMT_1

    Plasmid

    #106318

    Purpose

    Express Cas9 and sgRNA targeting MTFMT

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTFMT
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPRv2puro-Ptger1

    Plasmid

    #170306

    Purpose

    A knock-out vector for the mouse Ptger1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Ptger1 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. M-ST1-VPR

    Plasmid

    #68498

    Purpose

    nuclease competent ST1-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    ST-Cas9-VPR
    

    Tags

    VPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSECB sgMTHFD2L_1

    Plasmid

    #106306

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2L (MTHFD2L Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSECB sgMTHFD2L_2

    Plasmid

    #106307

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2L (MTHFD2L Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiCRISPRv2puro-Ptgs2_Balbc

    Plasmid

    #170301

    Purpose

    A knock-out vector for the mouse Ptgs2 in Balb/c

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgp27#1

    Plasmid

    #102759

    Purpose

    Expresses sgRNA targeting mouse p27

    Depositor

    Jin Chen

    Article

    Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJY-RpABE-FT_INF

    Plasmid

    #112874

    Purpose

    binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

    Depositor

    Jae-Young Yun, Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting FT
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti CRISPR sgSHMT1_2

    Plasmid

    #106312

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAT-9222

    Plasmid

    #124222

    Purpose

    Cloning plasmid for EGxFP assay with self-targeting gRNA-s

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA between EGFP halves
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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