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We narrowed to 63,826 results for: cloning

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Showing: 8521 - 8540 of 63826 results

  5. pHmK-C1

    Plasmid
    #87866
    Purpose
    Backbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1
    Depositor
    Isei Tanida
    Publication
    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pHluorin-mKate2 fusion
    Tags
    pHluorin-mKate2 fusion
    Expression
    Mammalian
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    87866-plasmid-map-sequence-id-252335

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  6. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

    Plasmid
    #111389
    Purpose
    AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Publication
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    ChETA-mRuby2
    W3SL
    Use
    AAV
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    W3SL is built from a shortened WPRE, an upstream …
    Promoter
    hSynapsin
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111389-plasmid-map-sequence-id-212217

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  7. pRVL-3

    Plasmid
    #104581
    Purpose
    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Publication
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and E318A/K320A/K322A
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    104581-plasmid-map-sequence-id-201392

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  8. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Publication
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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Showing: 8521 - 8540 of 63826 results