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pCW57-MCS1-P2A-MCS2 (GFP)

Plasmid

#80924

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP1

Plasmid

#52519

Purpose

For mammalian expression of the fluorescent voltage sensor ASAP1

Depositor

Michael Lin

Article

St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

Insert

Accelerated Sensor of Action Potentials 1

Expression

Mammalian

Promoter

CAG

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BYG

Plasmid

#158126

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_30bpLinker

Plasmid

#157789

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_30bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-ZETAipi

Plasmid

#53284

Purpose

Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

pds

Use

Low copy number bacterial cloning vector

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEN-X-A-ccdB-G-Z

Plasmid

#218892

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-C1

Plasmid

#60560

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pTIR_Poetur_RFP

Plasmid

#248202

Purpose

Poetur RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211463

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-dead

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EcoHIV-mScarlet

Plasmid

#235598

Purpose

EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env

Depositor

Michael Shtutman

Article

Li et al J Vis Exp. 2024 Dec 20;(214). doi: 10.37

Insert

NL4-3 EcoHIV mScarlet

Use

Lentiviral

Tags

mScarlet

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPdelta

Plasmid

#13394

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPdelta (Cebpd Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-synORP1

Plasmid

#191759

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-synORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-LMNA-gRNA1

Plasmid

#122507

Purpose

expresses WT spCas9 and a chimeric gRNA targeting human LMNA

Depositor

Graham Dellaire

Article

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

LMNA-gRNA1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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hTLR3-pcDNA3

Plasmid

#32712

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

TLR3 (TLR3 Human)

Tags

FLAG and Preprototrypsin

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pTagRFP-C1-Fibrillarin

Plasmid

#70649

Purpose

Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

Fibrillarin (FBL Human)

Tags

TagRFP

Expression

Mammalian

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag-JunB

Plasmid

#29687

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

jun B oncogene (JUNB Human)

Tags

Flag

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pSQT834

Plasmid

#53371

Purpose

Csy4 and Cas9 nuclease expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Csy4-T2A-Cas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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EcSTH-LbNOX

Plasmid

#211924

Purpose

This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

EcSTH-LbNOX

Use

Lentiviral

Tags

3xFLAG

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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