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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,634 results for: SEC

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Showing: 8561 - 8580 of 10634 results

  3. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

    Plasmid
    #169551
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Expression
    Mammalian
    Promoter
    PPGK
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169551-plasmid-map-sequence-id-364981

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  4. pMT-FLAG-CBPΔ(N+HAT)

    Plasmid
    #182841
    Purpose
    An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    CBP (w Fly)
    Tags
    FLAG tag
    Expression
    Insect
    Mutation
    dletion of residues 1566-2142 from CBP1476-3222
    Promoter
    MT
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182841-plasmid-map-sequence-id-360051

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  5. pGG198

    Plasmid
    #165605
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Secondary protospacer ('CGGCG' PAM) ins…
    Promoter
    lac
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    165605-plasmid-map-sequence-id-338038

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  10. pKM33

    Plasmid
    #154167
    Purpose
    Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Rel2 (long isoform)
    Tags
    FLAG
    Expression
    Insect
    Mutation
    C-terminal IκB domain deleted
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154167-plasmid-map-sequence-id-309408

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  12. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

    Plasmid
    #124068
    Purpose
    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Article
    He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1
    Insert
    eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
    Use
    CRISPR and Cre/Lox
    Expression
    Insect
    Promoter
    bhsp68
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124068-plasmid-map-sequence-id-240659

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  16. pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

    Plasmid
    #83458
    Purpose
    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)
    Depositor
    Thomas Weimbs
    Article
    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do
    Insert
    Polycystin-1 (PKD1 Human)
    Tags
    6xHis and Myc
    Expression
    Mammalian
    Mutation
    Amino-acids 4127-4303, S4144D
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83458-plasmid-map-sequence-id-159102

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  19. pUAST-HA, Pbl-A DH domain

    Plasmid
    #69794
    Purpose
    Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    van Impel et al Development. 2009 Mar;136(5):813-22. doi
    Insert
    Pebble DH domain
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    Contains only the Pebble DH domain
    Promoter
    hsp70
    Available Since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 8561 - 8580 of 10634 results