We narrowed to 19,051 results for: Tec

1. pCAG-BRCA1-Rad18UBD

   Plasmid

   #119008

   Purpose

   Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   BRCA1 (BRCA1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

   Plasmid

   #123354

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   cp-moxCerulean3-moxCerulean3-FLARE-D1ER
   

   Tags

   C-terminal ER retention signal (KDEL), cp-moxCeru…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCHKO_HPRT1_exon3_1_As

   Plasmid

   #155057

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCHKO_HPRT1_exon3_2_As

   Plasmid

   #155058

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCHKO_HPRT1_exon2_2_As

   Plasmid

   #155056

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCHKO_HPRT1_exon2_1_As

   Plasmid

   #155055

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCHKO_HPRT1_exon2_2_Lb

   Plasmid

   #155052

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Oct4deltaPE-53BP1mCherry

   Plasmid

   #141122

   Purpose

   Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

   Depositor

   John Schimenti

   Article

   Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

   Insert

   Fragment of human 53BP1 (TP53BP1 Human)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mCherry-mCherry-FLARE-CKAR

   Plasmid

   #123345

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-CKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR

    Plasmid

    #123335

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-AKAR
    

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA BRCA2 R2896C

    Plasmid

    #139511

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 R2896C using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 E2896C using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

    Plasmid

    #133796

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1 (truncated C-terminal domain only)
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    C-terminal domain only

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

    Plasmid

    #133798

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1(T114A/F115A)
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    T114A/F115A

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

    Plasmid

    #133791

    Purpose

    U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    St1Cas9 sgRNA entry vector
    

    Expression

    Mammalian

    Mutation

    V1 sgRNA architecture from Carter et al. biorxiv …

    Promoter

    U6

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid

    #133792

    Purpose

    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    St3Cas9 sgRNA entry vector
    

    Expression

    Mammalian

    Mutation

    sgRNA architecture from Muller et al. Molecular T…

    Promoter

    U6

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-HA-Flag-natT1R2 RR837-8GG

    Plasmid

    #113958

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839, R837G and R838G substitution

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNAS1b split mCherry NEDD4 WW2 neg ctrl

    Plasmid

    #112030

    Purpose

    Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Insert

    NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
    

    Tags

    Split mCherry

    Expression

    Bacterial

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. [pSK480] pLJC5 Flag-Depdc5(A)

    Plasmid

    #109338

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant A

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    DIYGD(185-189)GSGSG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. [pSK481] pLJC5 Flag-Depdc5(B)

    Plasmid

    #109339

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant B

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    EQPLH(371-375)GSGSG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. [pSK479] pLJC5 Flag-Depdc5(C)

    Plasmid

    #109340

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant C

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    FTPRI(419-423)GSGSG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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