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Showing: 2101 - 2150 of 2374 results

  6. pJL50

    Plasmid
    #174068
    Purpose
    Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    pTEF1-3NLS-97-3ZF-VP16
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    174068-plasmid-map-sequence-id-341751

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  10. pJL29

    Plasmid
    #174063
    Purpose
    Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pCyc1
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174063-plasmid-map-sequence-id-341950

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  12. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Article
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    210212-plasmid-map-sequence-id-416400

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  15. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  17. ZJOM151

    Plasmid
    #133643
    Purpose
    Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-COX4-GFP (COX4 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133643-plasmid-map-sequence-id-268961

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  18. ZJOM153

    Plasmid
    #133642
    Purpose
    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    Vph1-2GFP (VPH1 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133642-plasmid-map-sequence-id-268960

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  19. ZJOM31

    Plasmid
    #133645
    Purpose
    Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-TGL3-GFP (TGL3 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133645-plasmid-map-sequence-id-265890

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  22. ZJOM18

    Plasmid
    #133631
    Purpose
    Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-NAB2-GFP (NAB2 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133631-plasmid-map-sequence-id-270707

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  35. Gfap-Cryab

    Plasmid
    #58947
    Purpose
    hamster Cryab gene under the control of the human GFAP promoter to target expression to astrocytes
    Depositor
    Albee Messing
    Article
    Hagemann et al Hum Mol Genet. 2009 Apr 1;18(7):1190-9.
    Insert
    Cryab
    Use
    Mouse Targeting; Transgene expression
    Tags
    Expression
    Mammalian
    Mutation
    genomic fragment
    Promoter
    human GFAP promoter (position -2163 to +47)
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. pJL30

    Plasmid
    #174064
    Purpose
    Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    174064-plasmid-map-sequence-id-341957

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  49. pJL15

    Plasmid
    #174069
    Purpose
    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174069-plasmid-map-sequence-id-342040

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Showing: 2101 - 2150 of 2374 results