We narrowed to 25,015 results for: Nes

1. pCK411.RR1

   Plasmid

   #192644

   Purpose

   Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   BBa_J23119-RR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGI3EM29

   Plasmid

   #135489

   Purpose

   Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr hph-mCitrine
   

   Use

   Tags

   mCitrine

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGI3EM30

   Plasmid

   #135490

   Purpose

   Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr hph-mClover3
   

   Use

   Tags

   mClover3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGI3EM31

   Plasmid

   #135491

   Purpose

   Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr hph-mCerulean3
   

   Use

   Tags

   mCerulean3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNB786

   Plasmid

   #60151

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB682

   Plasmid

   #60143

   Purpose

   Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

   Plasmid

   #115684

   Purpose

   NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

   Depositor

   David Suter

   Article

   Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

   Insert

   Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDisplay-NE2m-IRES-mCherry-CAAX

   Plasmid

   #208690

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cyto-B

    Plasmid

    #209858

    Purpose

    Dimerization dependent fluorescent protein B fused to NES

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Syn1-mCherry

    Plasmid

    #222967

    Purpose

    Make lentivirus to express mCherry in syn1 expressing cells

    Depositor

    Xinyu Zhao

    Article

    Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    synapsin I

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDisplay-NE2h-IRES-mCherry-CAAX

    Plasmid

    #208691

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Cyto-ExRai-AktAR2

    Plasmid

    #184048

    Purpose

    Cytosol-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Cyto-ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM33_SUFU_Sufu

    Plasmid

    #178488

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    SUFU_Sufu (SUFU Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM33_SIN3A_PAH

    Plasmid

    #178486

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    SIN3A_PAH (SIN3A Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

    Plasmid

    #223174

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB-CAG-TEVp-IRES-mCherry

    Plasmid

    #174377

    Purpose

    For making TEVP cell line with hypBase

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    TEV Protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDN34

    Plasmid

    #61343

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS2 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-TREtight-EGFP

    Plasmid

    #182967

    Purpose

    For making cell lines for titering pRV∆L-5tTA virus

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EGFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRV∆L-5Cre

    Plasmid

    #182964

    Purpose

    The 3rd generation of rabies vector expressing Cre recombinase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    CRE recombinase
    

    Use

    Rabies

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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