We narrowed to 6,239 results for: nls

1. pEBG-hBEN-deltaNLS3

   Plasmid

   #22161

   Depositor

   Ananda Roy

   Article

   Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

   Insert

   hBEN (GTF2IRD1 Human)
   

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 883-889

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

2. TRE-mClover3-TDP-43ΔNLS

   Plasmid

   #214917

   Purpose

   inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   TDP-43ΔNLS (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Mutation

   mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-MBP-2xNLS-tdTomato

   Plasmid

   #104054

   Purpose

   An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-tdTomato
   

   Use

   AAV

   Tags

   NLS

   Promoter

   MBP

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRm-GFP(1-10)-VP64-NLS

   Plasmid

   #70228

   Purpose

   Expresses GFP(1-10)-VP64-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP(1-10)-VP64-NLS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-TurboCas-NLS-Blast

   Plasmid

   #232346

   Purpose

   Lentiviral expression vector for dCas9 fused to miniTurboNLS

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA, miniTurbo, V5 and 3xSV40 NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-miniTurbo-NLS-Blast

   Plasmid

   #232347

   Purpose

   Lentiviral control vector expressing miniTurboNLS without dCas9

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   miniTurbo
   

   Use

   Lentiviral

   Tags

   3xHA and 3xSV40 NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-puro-NLS-mCherry

   Plasmid

   #185474

   Purpose

   Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-BRX-eGFP-NLS

   Plasmid

   #217534

   Purpose

   AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   BMP reporter element and miniXon casette (Smad1 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   BMP Reporter Element

   Available Since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide-puro-NLS-GFP

   Plasmid

   #185473

   Purpose

   Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lp-BFPnls-LP-Cherry-T2A-NTR2.0

    Plasmid

    #219785

    Purpose

    Used for generating a transgenic line for inducing NTR2.0 expression in Cre positive cells after conversion

    Depositor

    Prayag Murawala

    Article

    García-García et al bioRxiv 2025.03.30.645595; doi: 10.1101/

    Insert

    mTagBFP2
    

    Tags

    3x SV40 NLS

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-EGFP-NLS

    Plasmid

    #165441

    Purpose

    Expresses nuclear EGFP from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    EGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Expression

    Mammalian

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CuO-LacI-NLS–SNAPf

    Plasmid

    #227368

    Purpose

    Lentiviral expression of LacI–SNAP

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    LacI NLS SNAPf
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid

    #117699

    Purpose

    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    

    Tags

    HA tag, Myc-NES, and NLS

    Expression

    Mammalian

    Mutation

    codon optimized for murine expression

    Promoter

    None, 2A peptide from porcine teschovirus-1 polyp…

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CSII-prEF1a-mCherry-3xNLS

    Plasmid

    #125262

    Purpose

    Fluorescent reporter for cell size

    Depositor

    Jan Skotheim

    Article

    Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

    Insert

    mCherry-3xNLS
    

    Use

    Lentiviral

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-XBP1 mNeonGreen NLS

    Plasmid

    #115968

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-ATF4 mScarlet NLS

    Plasmid

    #115969

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-EFS-MCP-3XBFPnls

    Plasmid

    #75384

    Purpose

    MCP-3XBFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    3XBFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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