We narrowed to 18,824 results for: nls
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Plasmid#46965PurposeExpresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissueDepositorInserthCas9
UseCRISPR; Plant expressionTagseGFPPromoter35SAvailable sinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-NLS-FRB-VP16
Plasmid#210515Purposeexpresses NLS-FRB-VP16 component in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-FRB-VP16
ExpressionMammalianPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a-P2A-tagBFP
Plasmid#84569PurposeLentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFPDepositorAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)
Plasmid#114073PurposeT7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R, S542R, K548R and N282AAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9m4-VP64
Plasmid#47319PurposeCas9m4 ActivatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9m4-VP64
UseCRISPRAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ920
Plasmid#42234DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPGK-Cre-bpA
Plasmid#11543PurposeExpresses Cre recombinase from mouse PGK promoter in mammalian cells.DepositorInsertPhosphoglycerate kinase 1 promoter
UseCre/LoxExpressionMammalianPromotermouse PGKAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWPT-GFP
Plasmid#12255DepositorInsertEGFP
UseCre/Lox and LentiviralExpressionMammalianAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TRE-dCas9-KRAB-MeCP2
Plasmid#122267PurposeSP-dCas9-KRAB-MeCP2 with doxycycline-inducible expressionDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-KRAB-MeCP2
TagsdCas9-KRAB-MeCP2ExpressionMammalianAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3XMEF2-luc
Plasmid#32967PurposeReporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMEF2 binding sites (MEF2A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterMEF2Available sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIRE
Plasmid#26745DepositorPublicationInsertiCre expression cassette
UseCre/Lox; Flp/frtExpressionMammalianAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQUAST
Plasmid#24349DepositorPublicationInsert5xQUAS-Pmin promoter
ExpressionInsectAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAd-PV-NLS-DsRed
Plasmid#17287DepositorAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCF140_Gag-SpyCas9-NLS
Plasmid#225959PurposeCMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV-Intron-Gag-SpyCas9-NLS
ExpressionMammalianAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-VP64 dCas9 VP64-T2A-GFP
Plasmid#59791PurposeCo-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFPDepositorInserthumanized VP64 dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840AAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IgK-IFN-luc
Plasmid#14886PurposeReporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIgK-IFN (IFNB1 Human)
UseLuciferaseExpressionMammalianAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPP441
Plasmid#158801PurposePlasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCasPhi-2
Tags2xSV40NLS-2xFLAGExpressionMammalianAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AIO-mCherry
Plasmid#74120PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by