We narrowed to 15,730 results for: NOL

1. CABE-T2.6

   Plasmid

   #193263

   Purpose

   Expression of CABE-T2.6 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T2.6
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CBE-T1.6

   Plasmid

   #193275

   Purpose

   Expression of CBE-T1.6 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.6
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Slap2-SH2

   Plasmid

   #46501

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Slap2 (SLA2 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 84-197

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC94

   Plasmid

   #118912

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and eGFP-HIS6

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX Lnk-SH2

   Plasmid

   #46451

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Lnk (Sh2b3 Mouse)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 325-466

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNAS1b split mCherry NEDD4-2 WW2

   Plasmid

   #111883

   Purpose

   Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Tags

   Split mCherry system: NEDD4-2 WW2 fused to C-term…

   Expression

   Bacterial

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. VRER variant

   Plasmid

   #138561

   Purpose

   Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRER
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM4889 (pCV0001)

   Plasmid

   #120079

   Purpose

   Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.

   Depositor

   Susan Golden

   Article

   Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

   Insert

   Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
   

   Use

   Other

   Mutation

   mobA Y25F

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. IBMc326

   Plasmid

   #161949

   Purpose

   Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVsc.U6-sgFah.CMV/CB-EGFP

    Plasmid

    #121508

    Purpose

    Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Intersectin I SH3 C domain (human)

    Plasmid

    #47415

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain C (ITSN1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain C

    Promoter

    tac

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. TAL2238

    Plasmid

    #36689

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD7-TALEN_Left (Chd7 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. TAL2239

    Plasmid

    #36690

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD7-TALEN_Right (Chd7 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pOCC254

    Plasmid

    #118889

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; mCherry, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTox-EGFPsite1_NGA-PAM (BPK754)

    Plasmid

    #181747

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
    

    Use

    Selection plasmid

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZ_P-GAP-LacI-Mit1AD

    Plasmid

    #126722

    Purpose

    LacI-Mit1AD on pGAPZ_ A expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-Mit1 activation domain coding sequence
    

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX p85b(NC)-SH2

    Plasmid

    #46468

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85b(NC) (PIK3R2 Bovine)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 311-717

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX Nck2-SH2

    Plasmid

    #46458

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    NM_001004720.2 (NCK2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 274-380

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUSEss-CHIg-mG3_F(ab')2

    Plasmid

    #105860

    Purpose

    Expression plasmid coding for heavy chain of mouse F(ab')2 of IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG3 F(ab')2 (Ighg3 Mouse)
    

    Expression

    Mammalian

    Promoter

    hEF1-HTLV prom

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX Tem6-SH2

    Plasmid

    #46523

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Tem6 (TNS3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1171-1291

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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