We narrowed to 9,776 results for: pho

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pHR-OptoCAR-mScarlet

Plasmid

#170463

Purpose

Expresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophore

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

OptoCAR

Use

Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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BMPR2

Plasmid

#38972

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BMPR2 (BMPR2 Human)

Tags

His6

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pHT-trxA.ads

Plasmid

#47383

Purpose

Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Insert

ads

Tags

6xHis and trxA

Expression

Bacterial

Promoter

Pgrac

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PAmKate

Plasmid

#32691

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Expression

Bacterial

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

araB

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-mCherry-Kan

Plasmid

#44950

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-PRSX8 eArchT3.0-EYFP

Plasmid

#89538

Purpose

Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Burke et al J Physiol. 2015 Jul 1;593(13):2909-26. d

Insert

eArchT 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

PRSx8

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-DHFR-myc

Plasmid

#118852

Purpose

For fluorophore translocation assay and inducible tagging with zapalog.

Depositor

Thomas Schwarz

Article

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Type

Empty backbone

Tags

DHFR, Myc, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-AsLOV2-NES27 (pDN158)

Plasmid

#72659

Purpose

Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-AsLOV2-NES27

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGGE001

Plasmid

#48839

Purpose

Provides the rbcS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

rbcS terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PTPN11

Plasmid

#38965

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTPN11 (PTPN11 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCAGGS-Chronos-mRuby2-ST

Plasmid

#105446

Purpose

Cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

Insert

Chronos-mRuby2-ST

Tags

mRuby2 and soma targeting motif

Promoter

pCAGGS

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-PA(WT)-Vav2

Plasmid

#86977

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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Chx10 3kb AP (CC#318)

Plasmid

#15205

Depositor

Connie Cepko

Article

Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

Insert

Chx10 3kb AP reporter (Vsx2 Mouse)

Expression

Bacterial

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yoPA-TagRFP-SpHis5

Plasmid

#44854

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-TagRFP

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPSmOrange-Kan

Plasmid

#44951

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPSmOrange

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pKL12-GCaMP6f-pHuji bb118

Plasmid

#158981

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-pHuji

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833 a…

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pME nls-eos

Plasmid

#73791

Purpose

Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

Depositor

David Raible

Article

Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

Insert

nls-eos

Use

Middle entry clone

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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Dronpa-Vinculin-23

Plasmid

#57308

Purpose

Localization: Focal Adhesions, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Vinculin (VCL Human)

Tags

Dronpa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFA6a-link-yoPA-mCherry-SpHis5

Plasmid

#44855

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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