We narrowed to 37,143 results for: Tec

1. SpCas9-NRCH-SsAPOBEC3B

   Plasmid

   #198550

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-SsAPOBEC3B
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mKate2-P2A-APEX2-2xFYVE_hrs

   Plasmid

   #67663

   Purpose

   Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-2xFYVE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_Xph20-GCaMP7f

   Plasmid

   #135539

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Tags

   GCaMP7f

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. eTd-CBEa

   Plasmid

   #196603

   Purpose

   Mammalian expression of eTd-CBEa

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PE2-tagRFP-BleoR

   Plasmid

   #192508

   Purpose

   Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

   Depositor

   Gerald Schwank

   Article

   Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   PE2-P2A-tagRFP-P2A-BleoR
   

   Tags

   BleoR, SV40 bpNLS, and tagRFP

   Expression

   Mammalian

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SFFV-HALO-CTERM-GFP

   Plasmid

   #185767

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, Halo

   Mutation

   none

   Promoter

   SFFV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TRE-DIO-GCaMP6s

   Plasmid

   #183809

   Purpose

   his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene TetO promoter-DIO/flex-GCaMP6
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAPM-miR30-DDX58-ts3

   Plasmid

   #174243

   Purpose

   DDX58 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   DDX58 shRNA (RIGI Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. A*24:07:01

    Plasmid

    #203283

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*24:07:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163533

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_UdgX-EE-UdgX-nCas9-UdgX

    Plasmid

    #163566

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

    Plasmid

    #123353

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-Cameleon
    

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-mA3-BE3

    Plasmid

    #113419

    Purpose

    Expresses mA3-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    mA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

    Plasmid

    #163543

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MuHKI-pGFPN3

    Plasmid

    #21919

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the full length human HKI cDNA sequence w…

    Available Since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid

    #123333

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-AKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-hA3G-BE3

    Plasmid

    #113415

    Purpose

    Expresses hA3G-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3G-BE3 (APOBEC3G Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJS647

    Plasmid

    #183176

    Purpose

    PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Insert

    PYR1 (PYR1 Mustard Weed)
    

    Expression

    Bacterial

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dCpf1-eBE

    Plasmid

    #107688

    Purpose

    Expresses dLbCpf1-eBE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE
    

    Expression

    Mammalian

    Mutation

    dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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