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pRS424-URR

Plasmid

#65330

Purpose

high copy number plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRS414-URR

Plasmid

#65329

Purpose

CEN plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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HcRed-pcw107

Plasmid

#64646

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Promoter

PGK

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only
pHBFD1

Plasmid

#53565

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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K-p35

Plasmid

#38788

Depositor

David Wang

Publication

Siebrasse et al J Virol. 2012 Jun 27.

Insert

4385-462 of MWPyV

Expression

Bacterial

Promoter

lac

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
pET-21a(+) SurE

Plasmid

#12669

Depositor

SeWon Suh

Publication

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE (TM1662 T.maritima)

Tags

His

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HI.HslUV.RSET.FL.wt

Plasmid

#12538

Depositor

David McKay

Publication

Sousa et al Cell. 2000 Nov 10. 103(4):633-43.

Insert

Haemophilus influenzae heat shock loci U and V

Expression

Bacterial

Mutation

none

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pET15-VraS - T331I

Plasmid

#206339

Purpose

Bacterial Expression of T331I VraS

Depositor

May Abdelaziz

Publication

Ali et al Microbiol Spectr. 2025 Apr 15;13(5):e000

Insert

VraS

Tags

6xHis

Expression

Bacterial

Mutation

T331I

Available since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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PCW57.1-ER-GshF-GFP

Plasmid

#257607

Purpose

Doxycycline-inducible expression of ER-targeted GshF-GFP

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

ER-GshF

Expression

Mammalian

Promoter

tight TRE promoter

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pjRGECO1a-Fb(LAG30)

Plasmid

#249313

Purpose

jRGECO1a-Fb(LAG30) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Publication

Barykina et al Nat Methods. 2026 Apr 22. doi: 10.1038/s

Insert

jRGECO1a-Fb(LAG30)

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pmScarlet-Fb(GFP)

Plasmid

#249298

Purpose

pmScarlet-Fb(GFP) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Publication

Barykina et al Nat Methods. 2026 Apr 22. doi: 10.1038/s

Insert

pmScarlet-Fb(GFP)

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pmNeonGreen-Fb(GFP)

Plasmid

#249294

Purpose

pmNeonGreen-Fb(GFP) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Publication

Barykina et al Nat Methods. 2026 Apr 22. doi: 10.1038/s

Insert

pmNeonGreen-Fb(GFP)

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Vega

Plasmid

#246323

Purpose

Photostable genetically encoded voltage indicator for imaging in HEK293T

Depositor

Peng Zou

Publication

Cao et al bioRxiv 2025.05.29.656768

Insert

Vega

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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EML8552_pEML41003-tufApst4-15

Plasmid

#237865

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufA

Depositor

Erh-Min Lai

Publication

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufA

Available since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV2ST

Plasmid

#239400

Purpose

An AAV entry vector with stabilized ITRs, permits packaging of desired inserts in cis.

Depositor

Fred Bunz

Insert

Green fluorescent protein

Use

AAV

Promoter

CMV

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPB.DEST-Illusia

Plasmid

#215444

Purpose

Piggybac expression vector with Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia

Use

Transposon-based stable expression

Expression

Mammalian

Promoter

CAG

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231364

Purpose

Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minCMVe-SCP1-SaCas9-W3SynpA

Plasmid

#231370

Purpose

SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

SaCas9

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Lysosome-N-20

Plasmid

#231770

Purpose

Visualization of lysosome in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Lysosome-N-20 (Lamp1 Rat)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH72

Plasmid

#202487

Purpose

Expresses the nanobody VHH72 with T7 induction

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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