166,671 results

1. pMSCV40-EF1a-hBCL10-SO

   Plasmid

   #75256

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Mutation

   Promoter

   pMSCV-LTRs, EF1a

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rogi1-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179864

   Purpose

   Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

   Plasmid

   #153006

   Purpose

   Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   OMM (Tom20)-sTurboID (N) (L73)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET Nucleolin

   Plasmid

   #13037

   Depositor

   Peter Newburger

   Article

   Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

   Insert

   Nucleolin (NCL Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0042-PguCas13b crRNA backbone

   Plasmid

   #103853

   Purpose

   For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-PguCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-FLuc+GFPd2

   Plasmid

   #127190

   Purpose

   Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)

   Depositor

   Jordan Green

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW SpCas9

   Plasmid

   #131506

   Purpose

   lentiviral expression of SpCas9 under hUBC promotor

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   SpCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBC

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

   Plasmid

   #216153

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic splice site and C-terminal FLAG tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

    Plasmid

    #195548

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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