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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,662 results for: Met

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Showing: 861 - 880 of 19662 results

  5. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  6. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  7. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  14. pET30a-SSDH

    Plasmid
    #239573
    Purpose
    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Article
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239573-plasmid-map-sequence-id-476594

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  20. pJBEI-6411

    Plasmid
    #47050
    Purpose
    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    Use
    Synthetic Biology; Bglbrick
    Expression
    Bacterial
    Promoter
    Pbad
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47050-plasmid-map-sequence-id-67072

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Showing: 861 - 880 of 19662 results