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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Tags

His6 and TEV site

Expression

Bacterial

Promoter

T5

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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fli1aCreERT2

Plasmid

#108639

Purpose

fli1aCreERT2

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

fli1aCreERT2

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A

Plasmid

#28047

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6Xhis-human SUMO 3

Plasmid

#133772

Purpose

express 6Xhis- SUMO 3 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 3 (SUMO3 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHnCBS1D

Plasmid

#52065

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

Depositor

David Savage

Article

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D

Expression

Bacterial

Available Since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX iLOV

Plasmid

#26587

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 phiC31

Plasmid

#68310

Purpose

In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

Depositor

Konrad Basler

Article

Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

Insert

phiC31

Use

Rna transcription

Promoter

CMV

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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acvr1l-HA

Plasmid

#201904

Purpose

in vitro transcription of RNA encoding HA-tagged Acvr1l

Depositor

Mary Mullins

Article

Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

acvr1l (acvr1l Zebrafish)

Tags

HA

Promoter

SP6

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEF6.mCherry-TSG101

Plasmid

#38318

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

TSG101 (TSG101 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCFD3-dU6:3gRNA

Plasmid

#49410

Purpose

expression of gRNA under control of the Drosophila U6:3 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-3

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh

Plasmid

#208276

Purpose

Expresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-CitA-Tq-HR

Plasmid

#241833

Purpose

Mammalian expression vector for CitA-Tq-HR

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

Insert

CitA-Tq-HR

Use

AAV

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-CitA-Tq-LR

Plasmid

#241832

Purpose

Mammalian expression vector for CitA-Tq-LR (low-range)

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

Insert

CitA-Tq-LR

Use

AAV

Expression

Mammalian

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP2

Plasmid

#59037

Purpose

for baculovirus expression of CAMSAP2 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pLNCX2-CFP-VGPDFGR-YFP

Plasmid

#59587

Purpose

Encodes a granzyme B FRET reporter

Depositor

Tim Mitchison

Article

Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

Insert

ECFP-VGPDFGR-Venus

Use

Retroviral

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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Pax2

Plasmid

#35002

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Paired box protein Pax-2 (Pax2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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