We narrowed to 932 results for: cas12a

1. pRDA52_Lenti.puro.U6_modified DR

   Plasmid

   #196723

   Purpose

   For cloning pairs of AsCas12a guides with two DRs that are as different as possible.

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. act-AsCpf1

   Plasmid

   #73917

   Purpose

   expression plasmid of AsCpf1

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   hAsCpf1
   

   Use

   Tags

   NLS-3xHA

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available Since

   March 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK517

   Plasmid

   #155385

   Purpose

   PphlF-TA4 in crRNAs

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Insert

   PphlF-TA4 in dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1.509

   Plasmid

   #203955

   Purpose

   Level 1 self-replicating vector containing a DAPG inducible FnCas12a

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Insert

   PphlF-Cpf1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p33Lb

   Plasmid

   #158156

   Purpose

   Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. enAsABE8e

   Plasmid

   #138506

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-dAsCas12a (En)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LbABE8e

   Plasmid

   #138504

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-dLbCas12a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL-his-LbCpf1-EC

   Plasmid

   #79008

   Purpose

   Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   LbCpf1
   

   Use

   Tags

   MBP-6xHis-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFnCpfAb-apr

   Plasmid

   #214198

   Purpose

   Expresses FnCpf1 and RecAb recombination system for genome editing in Acinetobacter baumannii

   Depositor

   Yu Wang

   Article

   Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   tac promoter

   Available Since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUbi-MAD7

    Plasmid

    #170133

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    maize Ubiquitin-1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIBR004

    Plasmid

    #177667

    Purpose

    SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ220 (AsCpf1)

    Plasmid

    #86208

    Purpose

    AsCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    AsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIBR003

    Plasmid

    #177666

    Purpose

    SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ5135: pCAG-BFP-GuideArray

    Plasmid

    #210606

    Purpose

    Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    pCAG-BFP-GuideArray
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p33Fn

    Plasmid

    #158155

    Purpose

    Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Rice codon optimized

    Promoter

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSIBR002

    Plasmid

    #177665

    Purpose

    SIBR Intron 2 (Int2) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTol2-HybridBarcode

    Plasmid

    #174037

    Purpose

    For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    CRISPR target sites
    

    Use

    Tol2 transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSIBR005

    Plasmid

    #177668

    Purpose

    Constitutive expression of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSIBR001

    Plasmid

    #177628

    Purpose

    SIBR Intron 1 (Int1) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge