We narrowed to 2,116 results for: pam

1. p35S::Cf-4-YFPc

   Plasmid

   #86160

   Purpose

   Cf-4 slplit YFP

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   Cf-4
   

   Use

   Tags

   YFPc

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p35S::AtSOBIR1-mCherry

   Plasmid

   #86166

   Purpose

   SOBIR1-mCherry expression

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   SOBIR1 (SOBIR1 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST_140_LVL2 cam

   Plasmid

   #179334

   Purpose

   NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA299

   Plasmid

   #216029

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Slug]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA296

   Plasmid

   #216028

   Purpose

   Fragmid fragment: (Cas protein) NNNNCC PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Nme2Cas9_v1.1 [Nme2]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA301

   Plasmid

   #216030

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sauri]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTBL437 AAVS1 EQR sgRNA

   Plasmid

   #126447

   Purpose

   To cut the human AAVS1 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human AAVS1 safe-harbor locus with NGAG PAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. F11-R pEBio

   Plasmid

   #61486

   Purpose

   Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F11R (F11R Human)
   

   Use

   Tags

   enzymatic biotinylation sequence and rat CD4 d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSIP103: pTarget(CyCAST)

    Plasmid

    #200849

    Purpose

    Target plasmid containing CyPSP1 and attachement site for CyCAST.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Inserts

    PAM for CyCAST + CyPSP1
    tRNA-Leu
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHEE901-A3A

    Plasmid

    #229044

    Purpose

    nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

    Depositor

    Huawei Zhang

    Article

    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL436 HEK293site3EQR sgRNA

    Plasmid

    #126445

    Purpose

    To cut the human HEK293 site3 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human HEK293 site3 with NGAG PAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p35S::SISOBIR1-YFPc

    Plasmid

    #86164

    Purpose

    SOBIR1 split YFP

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1 (SOBIR1 Mustard Weed)
    

    Use

    Tags

    YFPc

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p35S::AtSOBIR1-YFPn

    Plasmid

    #86165

    Purpose

    SOBIR1 split YFP

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1 (SOBIR1 Mustard Weed)
    

    Use

    Tags

    YFPn

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-2T-FRB-W2027F

    Plasmid

    #73341

    Purpose

    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    

    Use

    Tags

    GST-tagged

    Expression

    Bacterial

    Mutation

    FRB domain only aa2015-2114; W2027F

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-2T FRB Y2105A

    Plasmid

    #68726

    Purpose

    Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    

    Use

    Tags

    GST-tagged

    Expression

    Bacterial

    Mutation

    FRB domain only aa2015-2114; Y2105A

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

17. VE-cad KI gRNA1

    Plasmid

    #92310

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA1 (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. VE-cad KI gRNA2

    Plasmid

    #92311

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA2 (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA025

    Plasmid

    #216005

    Purpose

    Fragmid fragment: (Cas protein) NG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-NG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs_FLAG-MCART1

    Plasmid

    #133247

    Purpose

    Express FLAG-tagged MCART1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells and…

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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