We narrowed to 5,008 results for: puc

1. pUC18T-mini-Tn7T-hph-Ptol

   Plasmid

   #218369

   Purpose

   Empty backbone for Tn7-site insertion with XylS-Pm expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-TgKI-MCS-mLanYFP-polyA

   Plasmid

   #174021

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   mLanYFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

   Plasmid

   #111260

   Purpose

   mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   DsRed-Express2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pc

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC-DEST-cjBLIMP1-Venus-HygTK

   Plasmid

   #141028

   Purpose

   Reporter integration

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   mVenus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC57-Rluc-BoxB-A114-N40

   Plasmid

   #50562

   Purpose

   Template DNA for in vitro transcription using T7 polymerase

   Depositor

   Yukihide Tomari

   Article

   Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

   Insert

   Luciferase-BoxB-A114-N40
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19, containing H2-Kb c57/BL6 WK9

   Plasmid

   #102641

   Purpose

   Encodes sequence of the murine H2-Kb allele

   Depositor

   Sai Reddy

   Article

   Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

   Insert

   H2-Kd Balb/c
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19-P70a-Top-T~P70a-Bottom-T

   Plasmid

   #87314

   Purpose

   Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-Split-Broccoli-Toehold-Switch

   Plasmid

   #87316

   Purpose

   Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC57 DYSF, alt exon 1, 5a, 40a

   Plasmid

   #87889

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1, 5a, 40a (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIG-709pUC19-tNGFR-P2A-IL10

    Plasmid

    #186117

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL10 (IL10 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIG-746pUC19-tNGFR-P2A-CD5

    Plasmid

    #186121

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD5 (CD5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIG-738pUC19-tNGFR-P2A-Kindlin-3

    Plasmid

    #186092

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    KINDLIN3 (FERMT3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid

    #133793

    Purpose

    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    CjeCas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V2-TGAA linker sgRNA architecture from Kim et al.…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIG-707pUC19-tNGFR-P2A-TTC37

    Plasmid

    #186122

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    TTC37 (SKIC3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIG-736pUC19-tNGFR-P2A-CD40L

    Plasmid

    #186104

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD40L (CD40LG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCHR-CD5Flag2-P2A-smAID-mClover

    Plasmid

    #110657

    Purpose

    Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CD5Flag2-P2A-AID-mClover
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid

    #160437

    Purpose

    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Nematode, Human)
    

    Use

    Tags

    Cleavage signal

    Expression

    Bacterial

    Mutation

    Codon-optimized for expression in C. elegans

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57-Rluc-let-7-A114-N40

    Plasmid

    #50563

    Purpose

    Template DNA for in vitro transcription using T7 polymerase

    Depositor

    Yukihide Tomari

    Article

    Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

    Insert

    Luciferase-let7-A114-N40
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57 DYSF, alt exon 1a, 5a, 40a

    Plasmid

    #87897

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a, 5a, 40a (DYSF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19_msfGFP-NPM1-repair-template for U2OS

    Plasmid

    #238246

    Purpose

    For knock-in msfGFP to NPM1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NPM1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge