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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,774 results for: BLI

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Showing: 8781 - 8800 of 9774 results

  1. An R57A, R59A CAAX pMT2

    Plasmid
    #206119
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206119-plasmid-map-sequence-id-415935

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  2. An R57A, R59A HA CAAX pMT2

    Plasmid
    #206126
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Oct. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    206126-plasmid-map-sequence-id-412911

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  6. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid
    #194191
    Purpose
    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    4 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194191-plasmid-map-sequence-id-381776

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  7. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

    Plasmid
    #194192
    Purpose
    Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    3 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194192-plasmid-map-sequence-id-381933

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  12. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Expression
    Mammalian
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124230-plasmid-map-sequence-id-240604

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  13. BIC-Gag-CAS9

    Plasmid
    #119942
    Purpose
    encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.
    Depositor
    Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci
    Article
    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10
    Insert
    GAG FMLV fused to spCAS9
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    hCMV
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119942-plasmid-map-sequence-id-235075

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  15. pFP.E222

    Plasmid
    #138248
    Purpose
    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138248-plasmid-map-sequence-id-273451

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  16. pGBdest-GST-PI3Kc1

    Plasmid
    #187992
    Purpose
    Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326
    Depositor
    Sascha Martens
    Inserts
    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    Tags
    GST
    Expression
    Bacterial and Insect
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187992-plasmid-map-sequence-id-374167

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  18. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

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Showing: 8781 - 8800 of 9774 results