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EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

Plasmid

#204810

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available Since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLN426 (FuGW-G5p-mKate2)

Plasmid

#105183

Purpose

Module 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G5p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204790

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro

Expression

Bacterial

Mutation

MSAVMQ/SGLVKM

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MERS Mpro - His6-SUMO with autoprocessing - MSAVLQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204814

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro (orf1ab MERS)

Expression

Bacterial

Mutation

MSAVLQ/SGLVKM

Available Since

Feb. 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

Plasmid

#204803

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pnYC-NpM-DmRoquin_702-819-del725-755-del790-812-GB1His6_AE

Plasmid

#148621

Purpose

Bacterial Expression of DmRoquin_702-819-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del725-755-del790-812 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

Plasmid

#159557

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

G13D

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X32-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R

Plasmid

#159552

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R

Plasmid

#159559

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

Q61R

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X29-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V

Plasmid

#159549

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12V

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

Plasmid

#159555

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

G12D

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105209

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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R620-M04-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP N501Y

Plasmid

#170190

Purpose

ammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

3C-His8-SBP

Expression

Mammalian

Mutation

N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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Mode #2 Library (NEDD4-2 WW2 domain)

Pooled Library

#111708

Purpose

Encodes NEDD4-2 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (NEDD4 WW2 domain)

Pooled Library

#111707

Purpose

Encodes NEDD4 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (14-3-3σ)

Pooled Library

#111706

Purpose

Encodes 14-3-3σ and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
Mode #2 Library (14-3-3β)

Pooled Library

#111705

Purpose

Encodes 14-3-3β and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
L4278 hIgKLSS_hTGFb1 in pcDNA backbone

Plasmid

#244184

Purpose

Constitutive expression of human TGF-beta1 (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TGF-beta1 with IgK light chain secretion sequence (TGFB1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4275 hIgKLSS_hTNF in pcDNA backbone

Plasmid

#244182

Purpose

Constitutive expression of human mature TNF (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TNF with IgK light chain secretion sequence (TNF Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4437 x6TRE (cJun and AP-1) Ben DsRed-Express2 in TUPV1

Plasmid

#244197

Purpose

Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (AP-1 binding sites)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

cJun responsive promoter (6 AP-1 binding sites + YB_TATA), dsRedExpress2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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